LOCUS       BBa_K1124215             965 bp    DNA     linear       22-Dec-2024
DEFINITION  pLux-mCherry-LVA
ACCESSION   BBa_K1124215
VERSION     BBa_K1124215.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..75
                     /label=B0034
     CDS             82..828
                     /label=mCherry-LVA
     misc_feature    82..84
                     /label=Start
     misc_feature    793..822
                     /label=ssrA degradation tag: LVA
     misc_feature    823..825
                     /label=Stop
     misc_feature    826..828
                     /label=Stop
     misc_feature    64..828
                     /label=BBa_K773003
     stem_loop       848..891
                     /label=stem_loop
     misc_feature    837..916
                     /label=BBa_B0010
     terminator      837..916
                     /label=BBa_B0010
     stem_loop       932..951
                     /label=T7 TE
     misc_feature    958..958
                     /label=stop
     polyA_site      952..965
                     /label=polya
     misc_feature    925..965
                     /label=BBa_B0012
     terminator      925..965
                     /label=BBa_B0012
     terminator      837..965
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc
      121 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      181 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      241 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      301 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      361 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      421 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      481 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      541 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      601 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      661 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      721 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      781 ctgtacaagg cagcaaacga cgaaaactac gctctagtag cttaataata ctagagccag
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      961 ttata
//