LOCUS       BBa_K1124216             917 bp    DNA     linear       22-Nov-2024
DEFINITION  pLac-amilGFP
ACCESSION   BBa_K1124216
VERSION     BBa_K1124216.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..777
                     /label=amilGFP
     CDS             82..780
                     /label=BBa_K592010
     stem_loop       800..843
                     /label=stem_loop
     misc_feature    789..868
                     /label=BBa_B0010
     terminator      789..868
                     /label=BBa_B0010
     stem_loop       884..903
                     /label=T7 TE
     misc_feature    910..910
                     /label=stop
     polyA_site      904..917
                     /label=polya
     misc_feature    877..917
                     /label=BBa_B0012
     terminator      877..917
                     /label=BBa_B0012
     terminator      789..917
                     /label=BBa_B0015
     misc_feature    64..917
                     /label=BBa_K1124111
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtcttat tcaaagcatg gcatcgtaca agaaatgaag
      121 acgaaatacc atatggaagg cagtgtcaat ggccatgaat ttacgatcga aggtgtagga
      181 actgggtacc cttacgaagg gaaacagatg tccgaattag tgatcatcaa gcctgcggga
      241 aaaccccttc cattctcctt tgacatactg tcatcagtct ttcaatatgg aaaccgttgc
      301 ttcacaaagt acccggcaga catgcctgac tatttcaagc aagcattccc agatggaatg
      361 tcatatgaaa ggtcatttct atttgaggat ggagcagttg ctacagccag ctggaacatt
      421 cgtctcgaag gaaattgctt catccacaaa tccatctttc atggcgtaaa ctttcccgct
      481 gatggacccg taatgaaaaa gaagacaatt gactgggata agtccttcga aaaaatgact
      541 gtgtctaaag aggtgctaag aggtgacgtg actatgtttc ttatgctcga aggaggtggt
      601 tctcacagat gccaatttca ctccacttac aaaacagaga agccggtcac actgcccccg
      661 aatcatgtcg tagaacatca aattgtgagg accgaccttg gccaaagtgc aaaaggcttt
      721 acagtcaagc tggaagcaca tgccgcggct catgttaacc ctttgaaggt taaataataa
      781 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      841 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      901 gcctttctgc gtttata
//