##gff-version 3
##sequence-region BBa_K1124218 1 3122
BBa_K1124218	.	engineered_region	774	1434	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K1124218	.	ribosome_entry_site	774	785	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1124218	.	conserved_region	778	781	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124218	.	CDS	792	1409	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1124218	.	CDS	792	1370	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1124218	.	engineered_tag	1410	1434	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1124218	.	engineered_region	792	1409	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1124218	.	tag	1371	1402	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1124218	.	engineered_region	1443	2144	.	+	0	ID=BBa_S0107;Name=BBa_S0107
BBa_K1124218	.	ribosome_entry_site	1443	1453	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_S0107
BBa_K1124218	.	ribosome_entry_site	1443	1453	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1124218	.	engineered_region	1443	1453	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1124218	.	ribosome_entry_site	1449	1452	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1124218	.	CDS	1460	2119	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0107
BBa_K1124218	.	CDS	1463	2079	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1124218	.	tag	2080	2113	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1124218	.	engineered_tag	2120	2144	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1124218	.	engineered_region	1	765	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124218	.	start_codon	19	21	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124218	.	stop_codon	760	762	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124218	.	stop_codon	763	765	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124218	.	CDS	19	765	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124218	.	ribosome_entry_site	1	12	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124218	.	tag	730	759	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124218	.	engineered_region	2153	3122	.	+	0	ID=BBa_K516022;Name=BBa_K516022
BBa_K1124218	.	ribosome_entry_site	2153	2165	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1124218	.	ribosome_entry_site	2153	2165	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1124218	.	engineered_region	2153	2165	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1124218	.	ribosome_entry_site	2159	2162	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1124218	.	terminator	2994	3122	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1124218	.	terminator	2994	3073	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124218	.	engineered_region	2994	3073	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124218	.	stem_loop	3005	3048	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124218	.	terminator	3082	3122	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124218	.	engineered_region	3082	3122	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124218	.	polyA_site	3109	3122	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124218	.	stop_codon	3115	3115	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124218	.	stem_loop	3089	3108	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124218	.	CDS	2172	2960	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1124218	.	CDS	2172	2921	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1124218	.	stop_codon	2955	2960	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1124218	.	start_codon	2172	2174	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1124218	.	tag	2922	2954	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1124218	.	engineered_tag	2961	2985	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1124218	.	engineered_region	2172	2960	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
>BBa_K1124218
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaagg
tgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccca
gaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggc
tccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagc
gcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaa
ggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctcc
gagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacg
acgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgga
catcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatg
gacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagaaagaggagaaatacta
gatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttat
caagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatg
cagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatat
gctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtttt
gctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaac
acgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagt
tccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaat
gaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgct
agtgtagatctctactagagtcacacaggactactagatgtccagattagataaaagtaaagtgattaacagcgcatta
gagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacat
tgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcactt
ttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcat
cgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttt
tatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtatt
ggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaa
gctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaa
aacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgcta
gtgtagatcactactagagtcacacaggaaagtactagatgacagtaaagaagctttatttcgtcccagcaggtcgttg
tatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttg
gagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggta
catttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatga
gccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaataca
ccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatt
taaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagg
gcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaat
tttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtga
tgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagc
tgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata