LOCUS       BBa_K1124219            2412 bp    DNA     linear       22-Dec-2024
DEFINITION  mCherry-luxI-aiiA (+d.term)
ACCESSION   BBa_K1124219
VERSION     BBa_K1124219.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=B0034
     CDS             19..765
                     /label=mCherry-LVA
     misc_feature    19..21
                     /label=Start
     misc_feature    730..759
                     /label=ssrA degradation tag: LVA
     misc_feature    760..762
                     /label=Stop
     misc_feature    763..765
                     /label=Stop
     misc_feature    1..765
                     /label=BBa_K773003
     misc_feature    778..781
                     /label=conserved
     RBS             774..785
                     /label=BBa_B0034
     misc_feature    1371..1402
                     /label=LVA
     CDS             792..1370
                     /label=luxI
     misc_feature    792..1409
                     /label=BBa_C0061
     misc_feature    1410..1434
                     /label=Help:Barcodes
     CDS             792..1409
                     /label=BBa_C0061
     misc_feature    774..1434
                     /label=BBa_C0261
     RBS             1443..1455
                     /label=RBS-3\Weak
     RBS             1449..1452
                     /label=RBS
     misc_feature    1443..1455
                     /label=BBa_B0032
     RBS             1443..1455
                     /label=BBa_B0032
     misc_feature    1462..1464
                     /label=start
     misc_feature    2245..2250
                     /label=2
     misc_feature    2212..2244
                     /label=LVA
     CDS             1462..2211
                     /label=aiiA
     misc_feature    1462..2250
                     /label=BBa_C0060
     misc_feature    2251..2275
                     /label=Help:Barcodes
     CDS             1462..2250
                     /label=BBa_C0060
     stem_loop       2295..2338
                     /label=stem_loop
     misc_feature    2284..2363
                     /label=BBa_B0010
     terminator      2284..2363
                     /label=BBa_B0010
     stem_loop       2379..2398
                     /label=T7 TE
     misc_feature    2405..2405
                     /label=stop
     polyA_site      2399..2412
                     /label=polya
     misc_feature    2372..2412
                     /label=BBa_B0012
     terminator      2372..2412
                     /label=BBa_B0012
     terminator      2284..2412
                     /label=BBa_B0015
     misc_feature    1443..2412
                     /label=BBa_K516022
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
       61 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      181 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      241 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      301 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      361 gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      481 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
      541 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      601 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      661 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      721 tacaaggcag caaacgacga aaactacgct ctagtagctt aataatacta gagaaagagg
      781 agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg
      841 agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact
      901 tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt
      961 atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg
     1021 attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc
     1081 ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact
     1141 ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag
     1201 gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta
     1261 aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg
     1321 ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg
     1381 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag
     1441 agtcacacag gaaagtacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt
     1501 tgtatgttgg atcattcgtc tgttaatagt acattaacac caggagaatt attagactta
     1561 ccggtttggt gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg
     1621 ccagaaagtg cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt
     1681 ttaccgaaaa tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag
     1741 ccggaagacc ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat
     1801 ggcgctttta taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat
     1861 agcgaagaat atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt
     1921 gattatgaag tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat
     1981 caatcgctat taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg
     2041 tatacgaaag agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct
     2101 ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt
     2161 ggacatgata tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac
     2221 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
     2281 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2341 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2401 tctgcgttta ta
//