LOCUS       BBa_K1124220            2453 bp    DNA     linear       12-May-2024
DEFINITION  mCherry-tetR-aiiA (+d.term)
ACCESSION   BBa_K1124220
VERSION     BBa_K1124220.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=B0034
     CDS             19..765
                     /label=mCherry-LVA
     misc_feature    19..21
                     /label=Start
     misc_feature    730..759
                     /label=ssrA degradation tag: LVA
     misc_feature    760..762
                     /label=Stop
     misc_feature    763..765
                     /label=Stop
     misc_feature    1..765
                     /label=BBa_K773003
     RBS             774..784
                     /label=RBS-4\Weaker
     RBS             780..783
                     /label=RBS
     misc_feature    774..784
                     /label=BBa_B0033
     RBS             774..784
                     /label=BBa_B0033
     CDS             794..1410
                     /label=tetR
     misc_feature    1411..1444
                     /label=SsrA
     misc_feature    1451..1475
                     /label=Help:Barcodes
     CDS             791..1450
                     /label=BBa_C0040
     misc_feature    774..1475
                     /label=BBa_S0107
     RBS             1484..1496
                     /label=RBS-3\Weak
     RBS             1490..1493
                     /label=RBS
     misc_feature    1484..1496
                     /label=BBa_B0032
     RBS             1484..1496
                     /label=BBa_B0032
     misc_feature    1503..1505
                     /label=start
     misc_feature    2286..2291
                     /label=2
     misc_feature    2253..2285
                     /label=LVA
     CDS             1503..2252
                     /label=aiiA
     misc_feature    1503..2291
                     /label=BBa_C0060
     misc_feature    2292..2316
                     /label=Help:Barcodes
     CDS             1503..2291
                     /label=BBa_C0060
     stem_loop       2336..2379
                     /label=stem_loop
     misc_feature    2325..2404
                     /label=BBa_B0010
     terminator      2325..2404
                     /label=BBa_B0010
     stem_loop       2420..2439
                     /label=T7 TE
     misc_feature    2446..2446
                     /label=stop
     polyA_site      2440..2453
                     /label=polya
     misc_feature    2413..2453
                     /label=BBa_B0012
     terminator      2413..2453
                     /label=BBa_B0012
     terminator      2325..2453
                     /label=BBa_B0015
     misc_feature    1484..2453
                     /label=BBa_K516022
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
       61 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      181 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      241 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      301 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      361 gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      481 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
      541 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      601 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      661 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      721 tacaaggcag caaacgacga aaactacgct ctagtagctt aataatacta gagtcacaca
      781 ggactactag atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct
      841 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
      901 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
      961 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
     1021 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
     1081 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
     1141 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
     1201 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
     1261 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
     1321 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
     1381 agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt
     1441 agcttaataa cactgatagt gctagtgtag atcactacta gagtcacaca ggaaagtact
     1501 agatgacagt aaagaagctt tatttcgtcc cagcaggtcg ttgtatgttg gatcattcgt
     1561 ctgttaatag tacattaaca ccaggagaat tattagactt accggtttgg tgttatcttt
     1621 tggagactga agaaggacct attttagtag atacaggtat gccagaaagt gcagttaata
     1681 atgaaggtct ttttaacggt acatttgtcg aagggcaggt tttaccgaaa atgactgaag
     1741 aagatagaat cgtgaatatt ttaaaacggg ttggttatga gccggaagac cttctttata
     1801 ttattagttc tcacttgcat tttgatcatg caggaggaaa tggcgctttt ataaatacac
     1861 caatcattgt acagcgtgct gaatatgagg cggcgcagca tagcgaagaa tatttgaaag
     1921 aatgtatatt gccgaattta aactacaaaa tcattgaagg tgattatgaa gtcgtaccag
     1981 gagttcaatt attgcataca ccaggccata ctccagggca tcaatcgcta ttaattgaga
     2041 cagaaaaatc cggtcctgta ttattaacga ttgatgcatc gtatacgaaa gagaattttg
     2101 aaaatgaagt gccatttgcg ggatttgatt cagaattagc tttatcttca attaaacgtt
     2161 taaaagaagt ggtgatgaaa gagaagccga ttgttttctt tggacatgat atagagcagg
     2221 aaaggggatg taaagtgttc cctgaatata tagctgcaaa cgacgaaaac tacgctttag
     2281 tagcttaata acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa
     2341 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2401 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//