##gff-version 3
##sequence-region BBa_K1124220 1 2453
BBa_K1124220	.	engineered_region	1484	2453	.	+	0	ID=BBa_K516022;Name=BBa_K516022
BBa_K1124220	.	CDS	1503	2291	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1124220	.	stop_codon	2286	2291	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1124220	.	tag	2253	2285	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1124220	.	engineered_region	1503	2291	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1124220	.	start_codon	1503	1505	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1124220	.	CDS	1503	2252	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1124220	.	engineered_tag	2292	2316	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1124220	.	ribosome_entry_site	1484	1496	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1124220	.	ribosome_entry_site	1484	1496	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1124220	.	engineered_region	1484	1496	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1124220	.	ribosome_entry_site	1490	1493	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1124220	.	terminator	2325	2453	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1124220	.	terminator	2325	2404	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124220	.	stem_loop	2336	2379	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124220	.	engineered_region	2325	2404	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124220	.	terminator	2413	2453	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124220	.	polyA_site	2440	2453	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124220	.	stem_loop	2420	2439	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124220	.	stop_codon	2446	2446	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124220	.	engineered_region	2413	2453	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124220	.	engineered_region	1	765	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124220	.	stop_codon	760	762	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124220	.	start_codon	19	21	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124220	.	tag	730	759	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124220	.	ribosome_entry_site	1	12	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124220	.	stop_codon	763	765	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124220	.	CDS	19	765	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124220	.	engineered_region	774	1475	.	+	0	ID=BBa_S0107;Name=BBa_S0107
BBa_K1124220	.	ribosome_entry_site	774	784	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_S0107
BBa_K1124220	.	engineered_region	774	784	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1124220	.	ribosome_entry_site	774	784	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1124220	.	ribosome_entry_site	780	783	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1124220	.	CDS	791	1450	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0107
BBa_K1124220	.	engineered_tag	1451	1475	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1124220	.	CDS	794	1410	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1124220	.	tag	1411	1444	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
>BBa_K1124220
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaagg
tgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccca
gaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggc
tccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagc
gcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaa
ggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctcc
gagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacg
acgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgga
catcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatg
gacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagtcacacaggactactag
atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaa
cccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcga
cgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttta
cgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggccta
cagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattata
tgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagg
gaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccag
ccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacga
cgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacaggaaagtacta
gatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaaca
ccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggta
tgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactga
agaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttg
cattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgc
agcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgt
accaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggt
cctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcag
aattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatat
agagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataa
cgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tata