##gff-version 3
##sequence-region BBa_K1124221 1 1612
BBa_K1124221	.	engineered_region	1	765	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124221	.	CDS	19	765	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124221	.	ribosome_entry_site	1	12	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124221	.	start_codon	19	21	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124221	.	stop_codon	760	762	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124221	.	stop_codon	763	765	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124221	.	tag	730	759	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124221	.	terminator	1484	1612	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1124221	.	terminator	1484	1563	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124221	.	engineered_region	1484	1563	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124221	.	stem_loop	1495	1538	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124221	.	terminator	1572	1612	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124221	.	stop_codon	1605	1605	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124221	.	polyA_site	1599	1612	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124221	.	stem_loop	1579	1598	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124221	.	engineered_region	1572	1612	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124221	.	engineered_region	774	1475	.	+	0	ID=BBa_S0107;Name=BBa_S0107
BBa_K1124221	.	ribosome_entry_site	774	784	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_S0107
BBa_K1124221	.	ribosome_entry_site	774	784	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1124221	.	engineered_region	774	784	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1124221	.	ribosome_entry_site	780	783	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1124221	.	CDS	791	1450	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0107
BBa_K1124221	.	engineered_tag	1451	1475	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1124221	.	CDS	794	1410	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1124221	.	tag	1411	1444	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
>BBa_K1124221
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaagg
tgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccca
gaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggc
tccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagc
gcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaa
ggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctcc
gagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacg
acgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgga
catcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatg
gacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagtcacacaggactactag
atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaa
cccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcga
cgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttta
cgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggccta
cagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattata
tgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagg
gaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccag
ccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacga
cgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata