##gff-version 3
##sequence-region BBa_K1124222 1 1743
BBa_K1124222	.	engineered_region	1	765	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124222	.	stop_codon	760	762	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124222	.	start_codon	19	21	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124222	.	ribosome_entry_site	1	12	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124222	.	CDS	19	765	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124222	.	stop_codon	763	765	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124222	.	tag	730	759	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124222	.	engineered_region	774	1743	.	+	0	ID=BBa_K516022;Name=BBa_K516022
BBa_K1124222	.	CDS	793	1581	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1124222	.	engineered_tag	1582	1606	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1124222	.	tag	1543	1575	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1124222	.	start_codon	793	795	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1124222	.	CDS	793	1542	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1124222	.	stop_codon	1576	1581	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1124222	.	engineered_region	793	1581	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1124222	.	terminator	1615	1743	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1124222	.	terminator	1703	1743	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124222	.	polyA_site	1730	1743	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124222	.	stem_loop	1710	1729	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124222	.	stop_codon	1736	1736	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124222	.	engineered_region	1703	1743	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124222	.	terminator	1615	1694	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124222	.	stem_loop	1626	1669	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124222	.	engineered_region	1615	1694	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124222	.	ribosome_entry_site	774	786	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1124222	.	ribosome_entry_site	774	786	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1124222	.	ribosome_entry_site	780	783	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1124222	.	engineered_region	774	786	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_K1124222
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaagg
tgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccca
gaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggc
tccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagc
gcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaa
ggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctcc
gagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacg
acgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgga
catcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatg
gacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagtcacacaggaaagtact
agatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaac
accaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggt
atgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactg
aagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcactt
gcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcg
cagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcg
taccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccgg
tcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattca
gaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgata
tagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaata
acgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttata