LOCUS       BBa_K1124223            1680 bp    DNA     linear       10-May-2024
DEFINITION  tetR-aiiA (+d.term)
ACCESSION   BBa_K1124223
VERSION     BBa_K1124223.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=RBS-4\Weaker
     RBS             7..10
                     /label=RBS
     misc_feature    1..11
                     /label=BBa_B0033
     RBS             1..11
                     /label=BBa_B0033
     CDS             21..637
                     /label=tetR
     misc_feature    638..671
                     /label=SsrA
     misc_feature    678..702
                     /label=Help:Barcodes
     CDS             18..677
                     /label=BBa_C0040
     misc_feature    1..702
                     /label=BBa_S0107
     RBS             711..723
                     /label=RBS-3\Weak
     RBS             717..720
                     /label=RBS
     misc_feature    711..723
                     /label=BBa_B0032
     RBS             711..723
                     /label=BBa_B0032
     misc_feature    730..732
                     /label=start
     misc_feature    1513..1518
                     /label=2
     misc_feature    1480..1512
                     /label=LVA
     CDS             730..1479
                     /label=aiiA
     misc_feature    730..1518
                     /label=BBa_C0060
     misc_feature    1519..1543
                     /label=Help:Barcodes
     CDS             730..1518
                     /label=BBa_C0060
     stem_loop       1563..1606
                     /label=stem_loop
     misc_feature    1552..1631
                     /label=BBa_B0010
     terminator      1552..1631
                     /label=BBa_B0010
     stem_loop       1647..1666
                     /label=T7 TE
     misc_feature    1673..1673
                     /label=stop
     polyA_site      1667..1680
                     /label=polya
     misc_feature    1640..1680
                     /label=BBa_B0012
     terminator      1640..1680
                     /label=BBa_B0012
     terminator      1552..1680
                     /label=BBa_B0015
     misc_feature    711..1680
                     /label=BBa_K516022
ORIGIN
        1 tcacacagga ctactagatg tccagattag ataaaagtaa agtgattaac agcgcattag
       61 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
      121 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
      181 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
      241 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
      301 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
      361 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
      421 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
      481 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
      541 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
      601 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg
      661 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag tcacacagga
      721 aagtactaga tgacagtaaa gaagctttat ttcgtcccag caggtcgttg tatgttggat
      781 cattcgtctg ttaatagtac attaacacca ggagaattat tagacttacc ggtttggtgt
      841 tatcttttgg agactgaaga aggacctatt ttagtagata caggtatgcc agaaagtgca
      901 gttaataatg aaggtctttt taacggtaca tttgtcgaag ggcaggtttt accgaaaatg
      961 actgaagaag atagaatcgt gaatatttta aaacgggttg gttatgagcc ggaagacctt
     1021 ctttatatta ttagttctca cttgcatttt gatcatgcag gaggaaatgg cgcttttata
     1081 aatacaccaa tcattgtaca gcgtgctgaa tatgaggcgg cgcagcatag cgaagaatat
     1141 ttgaaagaat gtatattgcc gaatttaaac tacaaaatca ttgaaggtga ttatgaagtc
     1201 gtaccaggag ttcaattatt gcatacacca ggccatactc cagggcatca atcgctatta
     1261 attgagacag aaaaatccgg tcctgtatta ttaacgattg atgcatcgta tacgaaagag
     1321 aattttgaaa atgaagtgcc atttgcggga tttgattcag aattagcttt atcttcaatt
     1381 aaacgtttaa aagaagtggt gatgaaagag aagccgattg ttttctttgg acatgatata
     1441 gagcaggaaa ggggatgtaa agtgttccct gaatatatag ctgcaaacga cgaaaactac
     1501 gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc
     1561 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1621 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//