##gff-version 3
##sequence-region BBa_K1124223 1 1680
BBa_K1124223	.	engineered_region	711	1680	.	+	0	ID=BBa_K516022;Name=BBa_K516022
BBa_K1124223	.	CDS	730	1518	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1124223	.	engineered_tag	1519	1543	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1124223	.	CDS	730	1479	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1124223	.	tag	1480	1512	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1124223	.	stop_codon	1513	1518	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1124223	.	engineered_region	730	1518	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1124223	.	start_codon	730	732	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1124223	.	terminator	1552	1680	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1124223	.	terminator	1640	1680	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124223	.	engineered_region	1640	1680	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124223	.	stem_loop	1647	1666	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124223	.	polyA_site	1667	1680	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124223	.	stop_codon	1673	1673	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124223	.	terminator	1552	1631	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124223	.	engineered_region	1552	1631	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124223	.	stem_loop	1563	1606	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124223	.	ribosome_entry_site	711	723	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1124223	.	ribosome_entry_site	711	723	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1124223	.	engineered_region	711	723	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1124223	.	ribosome_entry_site	717	720	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1124223	.	engineered_region	1	702	.	+	0	ID=BBa_S0107;Name=BBa_S0107
BBa_K1124223	.	CDS	18	677	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0107
BBa_K1124223	.	CDS	21	637	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1124223	.	engineered_tag	678	702	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1124223	.	tag	638	671	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1124223	.	ribosome_entry_site	1	11	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_S0107
BBa_K1124223	.	ribosome_entry_site	1	11	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1124223	.	engineered_region	1	11	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1124223	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
>BBa_K1124223
tcacacaggactactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcgg
aatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaat
aagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaa
gctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtaca
tttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttca
ctagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaag
tcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatca
ccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagt
gggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagt
cacacaggaaagtactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctg
ttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctat
tttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggtt
ttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttata
ttattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgc
tgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaa
ggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattg
agacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatt
tgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgtt
ttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacg
ctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata