BBa_K112613
1
BBa_K112613
a~LamB pp> in BBb
2008-10-21T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of {a~LamB>} basic part K112613
PCR sc009/sc014 on MG1655 (111 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
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sc009 Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
sc014 Reverse biobricking of {prepro>}
ccagtGGATCCagccattgcctgagcagac
</pre>
This is a lamB prepro with an N4 spacer, an atg start codon, but no stop codon.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3386
9
It's complicated
true
N/A
false
Sherine Cheung
BBa_K112613_sequence
1
tagaatgatgattactctgcgcaaacttcctctggcggttgccgtcgcagcgggcgtaatgtctgctcaggcaatggct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z