BBa_K112601
1
BBa_K112601
< AP tag ! in BBb
2008-09-02T11:00:00Z
2015-05-08T01:09:18Z
Construction of {<AP!} basic part K112601
Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112601
--------------------------------------------------
SC001 Forward Biobricking of <AP-tag!
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC003 Reverse Biobricking of <AP-tag!
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
This is an AP tag with stop codon, but no start.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3386
9
It's complicated
true
N/A
false
Sherine Cheung
BBa_K112617
1
BBa_K112617
< HA!.b1006 in BBb
2008-10-21T11:00:00Z
2015-05-08T01:09:18Z
K112601 assembled with K112709
This is a composite part. AP tag with no start but a stop codon is the lefty parent of the part;
terminator b1006 is the righty parent of the part. It is in BBb format.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3386
9
Not in stock
false
N/A
false
Sherine Cheung
component1984048
1
BBa_K112601
component1984049
1
BBa_K112999
component1984050
1
BBa_K112709
annotation1984050
1
BBa_K112709
range1984050
1
55
94
annotation1984048
1
BBa_K112601
range1984048
1
1
48
annotation1984049
1
BBa_K112999
range1984049
1
49
54
BBa_K112709
1
BBa_K112709
b1006
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of <b1006> basic part K112709
Wobble PCR of dv021/dv022 (66bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+66, L)
Product is pBca1256-K112709
-----------------------------------------------
dv021 Forward biobricking of b1006
cgataGAATTCatgAGATCTaaaaaaaaaccccgcccctgacagggcgg
dv022 Reverse biobricking of b1006
GGATCCtaaaaaaaaccccgccctgtcaggggcggggtttttt
</pre>
The b1006 terminator.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
true
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112709_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttta
BBa_K112617_sequence
1
ggcctgaacgatatttttgaagcgcagaaaattgaatggcatgaataaggatctaaaaaaaaaccccgcccctgacagggcggggtttttttta
BBa_K112999_sequence
1
ggatct
BBa_K112601_sequence
1
ggcctgaacgatatttttgaagcgcagaaaattgaatggcatgaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z