BBa_K112709
1
BBa_K112709
b1006
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of <b1006> basic part K112709
Wobble PCR of dv021/dv022 (66bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+66, L)
Product is pBca1256-K112709
-----------------------------------------------
dv021 Forward biobricking of b1006
cgataGAATTCatgAGATCTaaaaaaaaaccccgcccctgacagggcgg
dv022 Reverse biobricking of b1006
GGATCCtaaaaaaaaccccgccctgtcaggggcggggtttttt
</pre>
The b1006 terminator.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
true
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112503
1
BBa_K112503
< myc ! in BBb
2008-10-21T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of {<myc!} basic part K112503
Wobble PCR of cc004/cc006 ( 54 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI,2227+ 910,L)
Product is pBca1256-K112503
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006 Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC
</pre>
This is a myc tag without a start, but with a stop codon. It is in BBb format.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3386
9
It's complicated
true
N/A
false
Sherine Cheung
BBa_K112619
1
BBa_K112619
{<myc!}.{b1006} in BBb
2008-10-21T11:00:00Z
2015-05-08T01:09:18Z
K112503 assembled with K112709
<myc!.b1006
This is a composite part. Myc tag with no start but a stop codon is the lefty parent of the part;
terminator b1006 is the righty parent of the part.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3386
9
Not in stock
false
N/A
false
Sherine Cheung
component1984055
1
BBa_K112999
component1984054
1
BBa_K112503
component1984056
1
BBa_K112709
annotation1984055
1
BBa_K112999
range1984055
1
34
39
annotation1984056
1
BBa_K112709
range1984056
1
40
79
annotation1984054
1
BBa_K112503
range1984054
1
1
33
BBa_K112709_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttta
BBa_K112503_sequence
1
gaacaaaaactcatctcagaagaggatctgtaa
BBa_K112619_sequence
1
gaacaaaaactcatctcagaagaggatctgtaaggatctaaaaaaaaaccccgcccctgacagggcggggtttttttta
BBa_K112999_sequence
1
ggatct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z