BBa_K112624
1
BBa_K112624
{Pbad}.{rbs_pelB>}.{<phoA>} in BBb
2008-10-21T11:00:00Z
2015-05-08T01:09:18Z
This part was made using AKL41, which consisted of {Pbad}.{rbs_pelB>}.{<phoA!}.{<b1006>}. The stop codon of the phoA region and the <b1006> terminator were removed.
PCR with ca998 Forward oligo, and mea37 reverse oligo for {<phoA>}.
This part is assembled with pBad promoter, rbs.pelB> (pelB prepro with native rbs and start codon, but no stop), and a <phoA> region (no start or stop codon).
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3386
9
Not in stock
false
N/A
false
Sherine Cheung
BBa_K112624_sequence
1
ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccggatctgacaccacaacaatatccgtactggataatcgtgcggcgcagggcgatattactacgcccggcggcgcgcgccgattaaccggcgatcaaacggcggcgctgcgtgaatcgctaaatgataaaccggcgaaaaatattattctgctgatcggcgacggaatgggagattctgaaataaccgccgcacgaaattatgccgaaggcgccggcggcttttttaaaggtattgatgccctgccgctgaccggacaatatacccactatgcgctgaataaaaaaaccggcaaaccagattacgtgaccgattccgccgcctcagccaccgcgtggtccacaggggtgaaaacctacaacggcgcgctgggggtcgatattcatgaaaaagatcatctgaccattctcgaaatggcgaaggccgcaggtctggctaccggcaacgtctcgaccgcggagttgcaggacgccaccccggcagcgctgattgcccatgtcacctcgcgcaaatgctatggcccgacggcgaccagcgaaaaatgtccgacaaacgcgctggaaaagggcggcaaaggctcgattactgaacagctcctgaacgcgcgggcggacgtcaccctgggcggcggcgcgaaaaccttcgccgagacggcaaccgccggcgagtggcaggggaaaacgctgcgtgagcaggcgcagatgcgcggctaccagttagtcggcgatgccgcctctttagcggcgatcgacgaagcgaatcaggacaaaccgctgctgggactgttcgcagaggggaatatgccggtgcgctgggaagggccgaaggcctcgtaccacggcaatattgataaacctgccgtgacctgtacgccaaatccgaaacgcaatgacgcgattcccacgctggcgcagatgaccgacaaagccatcgaactgttgagcaaaaatgagcggggcttctttttacaggtggaaggcgcgtctatcgataagcaggatcacgccgcgaacccgtgcggacagattggcgagacggtggatcttgatgaagccgtacagagggcgctggcctttgcgaagaaagacggcaatacgctggtgatcgttaccgccgatcatgctcatgccagccagatcgtggcgcccgaaacgaaagcgccggggctgacgcaggcgctgaacaccaaagatggcgcggtgatggtcatcagctacggcaactcggaagaagattcccaggagcataccggcagccagctgcgcatcgccgcttacgggccgcatgcggcgaacgtggtcgggctgaccgatcaaaccgatctgttctacaccatgaaagcggcgctgggcctgaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z