BBa_K1127008
1
BBa_K1127008
Gold sensing device (PgolTS+LacZ+GolS)
2013-09-04T11:00:00Z
2015-05-08T01:09:18Z
The part itself was synthesized fully [i]de novo[/i].
DNA sequence for PgolTS comes from Salmonela enterica as does the amino acid sequence for GolS (DNA sequence is different however).
This is the device we use in our project so that the cell could recognize the presence of gold in it's environment.
GolS protein acts as a positive transcriptional regulator on PgolTS once it binds to gold.
Part was designed so that you can detect and quantify the activity of the promoter via Beta-galactosidase assay. For convenience the ORF of LacZ is covered by restriction sites of SacII and SanDI which allows the cell to respond to gold by expressing something to meet your needs.
false
false
_1439_
0
16991
9
In stock
false
N/A
false
York iGEM 2013
annotation2334988
1
rbs
range2334988
1
272
283
annotation2334982
1
GFP wat?
range2334982
1
64
77
annotation2334981
1
SandI
range2334981
1
265
271
annotation2334977
1
PgolTS
range2334977
1
1
39
annotation2334979
1
rbs
range2334979
1
46
57
annotation2334989
1
GolS
range2334989
1
290
754
annotation2334978
1
SacII
range2334978
1
40
45
annotation2334980
1
lacZ-alpha
range2334980
1
78
263
BBa_K1127008_sequence
1
cttgaccttcccacaatggcaagctttaggctttctgatccgcggaaagaggagaaatactagatgaactatacaaaatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctaataaggggtcccaaagaggagaaaactagaatgaacatcggtaaagctgctaaagcttctaaagtttctgctaaaatgatccgttactacgaacagatcggtctgatcccggctgcttctcgtaccgactctggttaccgtgcttacacccaggctgacgttaaccagctgcacttcatccgtcgtgctcgtgacctgggtttctctgttgctgaaatctctgacctgctgaacctgtggaacaaccagtctcgtcagtctgctgacgttaaacgtctggctcagacccacatcgacgaactggaccgtcgtatccagaacatgcagcacatggctcagaccctgaaagctctgatccactgctgcgctggtgacgctctgccggactgcccgatcctgcacaccctgggtcagccggacgactctgaaccggaagctcgtaccggtgctgttctgcgtcgtccgcgtcgtcacggtctggctaaacgtctgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z