BBa_K112701 1 BBa_K112701 hns promoter 2008-10-20T11:00:00Z 2015-05-08T01:09:18Z <pre> Construction of [<Phns>] Basic Part K112701 PCR dv001/dv006 on Phns from E. coli K12 MG1655 (~342 bp, gp = A) PCR dv005/dv003 on Phns from E. coli K12 MG1655 (225 bp, gp = B) PCR dv004/dv002 on Phns from E. coli K12 MG1655 (~149 bp, gp = C) --------------------------------------------------- PCR dv001/dv003 on A+B (355 bp, gp = D) --------------------------------------------------- PCR dv001/dv002 on C+D (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+697) Product is pBca1256-K112701 ----------------------------------------------- dv001 Forward Biobricking of [<Phns>] CgATAgaattcATGagatctGAAATATAGCTGTGCCATCAGCC dv002 Reverse Biobricking of [<Phns>] cagtcggatccGCACGAAGAGTACGGATG dv003 Removing the 2nd EcoRI site in [<Phns>] (R) GCCAGGAATGTAAGGgATTCAAAATTGTTC dv004 Removing the 2ND EcoRI site in [<Phns>] (F) GAACAATTTTGAATcCCTTACATTCCTGGC dv005 Removing the FIRST EcoRI site in [<Phns>] (F)CGCTTAATAGGGgATTCTCGTAAACAC dv006 Removing the FIRST EcoRI site in [<Phns>] (R)GTGTTTACGAGAATcCCCTATTAAGCG </pre> Phns in BBb1 format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112701_sequence 1 gaaatatagctgtgccatcagccacaggccctcaatgatatgaaataaagttggctggagtttatcataattcgtcagtttttcagctcaacagtatgcacagaagtgggtatatgcgttctcccttacgaagccttgcataatccttctgagctatcattacaactgcctcgcttgttataagcgggtaaatgactgctggtaactattcacaatctttaacctgttgcgcaagtaatagccctctgttgacctccaggagatagtgcaatactaagtccatgctcttattgcgactgttctacttttcatcattcgcttaataggggattctcgtaaacacaactaatacagaagactgaaaggtcgtcagcctacgataatctccccataaaatgtgacatgaatcaggaagttttaacctcacgtgctgcgaaatcatcggtgtaaatagggctatatgccgcgtcttttctggctaattttatgaaaagatatttattggcggcacaaaataaagaacaattttgaatcccttacattcctggctattgcacaactgaatttaaggctctattattacctcaacaaaccaccccaatataagtttgagattactacaatgagcgaagcacttaaaattctgaacaacatccgtactcttcgtgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z