BBa_K112701
1
BBa_K112701
hns promoter
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of [<Phns>] Basic Part K112701
PCR dv001/dv006 on Phns from E. coli K12 MG1655 (~342 bp, gp = A)
PCR dv005/dv003 on Phns from E. coli K12 MG1655 (225 bp, gp = B)
PCR dv004/dv002 on Phns from E. coli K12 MG1655 (~149 bp, gp = C)
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PCR dv001/dv003 on A+B (355 bp, gp = D)
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PCR dv001/dv002 on C+D (697bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+697)
Product is pBca1256-K112701
-----------------------------------------------
dv001 Forward Biobricking of [<Phns>] CgATAgaattcATGagatctGAAATATAGCTGTGCCATCAGCC
dv002 Reverse Biobricking of [<Phns>] cagtcggatccGCACGAAGAGTACGGATG
dv003 Removing the 2nd EcoRI site in [<Phns>] (R) GCCAGGAATGTAAGGgATTCAAAATTGTTC
dv004 Removing the 2ND EcoRI site in [<Phns>] (F) GAACAATTTTGAATcCCTTACATTCCTGGC
dv005 Removing the FIRST EcoRI site in [<Phns>] (F)CGCTTAATAGGGgATTCTCGTAAACAC
dv006 Removing the FIRST EcoRI site in [<Phns>] (R)GTGTTTACGAGAATcCCCTATTAAGCG
</pre>
Phns in BBb1 format.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112701_sequence
1
gaaatatagctgtgccatcagccacaggccctcaatgatatgaaataaagttggctggagtttatcataattcgtcagtttttcagctcaacagtatgcacagaagtgggtatatgcgttctcccttacgaagccttgcataatccttctgagctatcattacaactgcctcgcttgttataagcgggtaaatgactgctggtaactattcacaatctttaacctgttgcgcaagtaatagccctctgttgacctccaggagatagtgcaatactaagtccatgctcttattgcgactgttctacttttcatcattcgcttaataggggattctcgtaaacacaactaatacagaagactgaaaggtcgtcagcctacgataatctccccataaaatgtgacatgaatcaggaagttttaacctcacgtgctgcgaaatcatcggtgtaaatagggctatatgccgcgtcttttctggctaattttatgaaaagatatttattggcggcacaaaataaagaacaattttgaatcccttacattcctggctattgcacaactgaatttaaggctctattattacctcaacaaaccaccccaatataagtttgagattactacaatgagcgaagcacttaaaattctgaacaacatccgtactcttcgtgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z