##gff-version 3
##sequence-region BBa_K1127016 1 3708
BBa_K1127016	.	engineered_region	1784	2719	.	+	0	ID=BBa_I0462;Name=BBa_I0462
BBa_K1127016	.	terminator	2591	2670	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K1127016	.	stem_loop	2602	2645	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1127016	.	engineered_region	2591	2670	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1127016	.	ribosome_entry_site	1784	1795	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K1127016	.	conserved_region	1788	1791	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1127016	.	terminator	2679	2719	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K1127016	.	engineered_region	2679	2719	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1127016	.	stop_codon	2712	2712	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1127016	.	polyA_site	2706	2719	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1127016	.	stem_loop	2686	2705	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1127016	.	CDS	1802	2557	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K1127016	.	point_mutation	2293	2293	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1127016	.	engineered_region	1802	2557	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1127016	.	engineered_region	1802	1803	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1127016	.	CDS	1802	2551	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1127016	.	point_mutation	1975	1975	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1127016	.	engineered_tag	2558	2582	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1127016	.	engineered_region	2911	3708	.	+	0	ID=BBa_K805016;Name=BBa_K805016
BBa_K1127016	.	ribosome_entry_site	2911	2922	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K805016
BBa_K1127016	.	conserved_region	2915	2918	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1127016	.	CDS	2929	3546	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K805016
BBa_K1127016	.	engineered_region	2929	3546	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1127016	.	engineered_tag	3547	3571	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1127016	.	tag	3508	3539	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1127016	.	CDS	2929	3507	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1127016	.	terminator	3580	3708	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K805016
BBa_K1127016	.	terminator	3580	3659	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1127016	.	stem_loop	3591	3634	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1127016	.	engineered_region	3580	3659	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1127016	.	terminator	3668	3708	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1127016	.	engineered_region	3668	3708	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1127016	.	stop_codon	3701	3701	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1127016	.	polyA_site	3695	3708	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1127016	.	stem_loop	3675	3694	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1127016	.	engineered_region	763	1732	.	+	0	ID=BBa_K516022;Name=BBa_K516022
BBa_K1127016	.	ribosome_entry_site	763	775	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1127016	.	ribosome_entry_site	769	772	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1127016	.	ribosome_entry_site	763	775	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1127016	.	engineered_region	763	775	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1127016	.	terminator	1604	1732	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1127016	.	terminator	1604	1683	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1127016	.	stem_loop	1615	1658	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1127016	.	engineered_region	1604	1683	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1127016	.	terminator	1692	1732	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1127016	.	engineered_region	1692	1732	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1127016	.	stop_codon	1725	1725	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1127016	.	polyA_site	1719	1732	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1127016	.	stem_loop	1699	1718	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1127016	.	CDS	782	1570	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1127016	.	stop_codon	1565	1570	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1127016	.	start_codon	782	784	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1127016	.	CDS	782	1531	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1127016	.	engineered_region	782	1570	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1127016	.	tag	1532	1564	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1127016	.	engineered_tag	1571	1595	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1127016	.	engineered_region	1	754	.	+	0	ID=BBa_K1127008;Name=BBa_K1127008
BBa_K1127016	.	ribosome_entry_site	46	57	.	+	0	ID=annotation2334979;Name=rbs;Parent=BBa_K1127008
BBa_K1127016	.	ribosome_entry_site	272	283	.	+	0	ID=annotation2334988;Name=rbs;Parent=BBa_K1127008
BBa_K1127016	.	CDS	78	263	.	+	0	ID=annotation2334980;Name=lacZ-alpha;Parent=BBa_K1127008
BBa_K1127016	.	sequence_feature	64	77	.	+	0	ID=annotation2334982;Name=GFP wat?;Parent=BBa_K1127008
BBa_K1127016	.	promoter	1	39	.	+	0	ID=annotation2334977;Name=PgolTS;Parent=BBa_K1127008
BBa_K1127016	.	sequence_feature	40	45	.	+	0	ID=annotation2334978;Name=SacII;Parent=BBa_K1127008
BBa_K1127016	.	sequence_feature	265	271	.	+	0	ID=annotation2334981;Name=SandI;Parent=BBa_K1127008
BBa_K1127016	.	CDS	290	754	.	+	0	ID=annotation2334989;Name=GolS;Parent=BBa_K1127008
BBa_K1127016	.	engineered_region	2728	2902	.	+	0	ID=BBa_K1127010;Name=BBa_K1127010
BBa_K1127016	.	promoter	1741	1775	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K1127016
cttgaccttcccacaatggcaagctttaggctttctgatccgcggaaagaggagaaatactagatgaactatacaaaat
gaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
cttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgca
gcctgaatggcgaatggcgctaataaggggtcccaaagaggagaaaactagaatgaacatcggtaaagctgctaaagct
tctaaagtttctgctaaaatgatccgttactacgaacagatcggtctgatcccggctgcttctcgtaccgactctggtt
accgtgcttacacccaggctgacgttaaccagctgcacttcatccgtcgtgctcgtgacctgggtttctctgttgctga
aatctctgacctgctgaacctgtggaacaaccagtctcgtcagtctgctgacgttaaacgtctggctcagacccacatc
gacgaactggaccgtcgtatccagaacatgcagcacatggctcagaccctgaaagctctgatccactgctgcgctggtg
acgctctgccggactgcccgatcctgcacaccctgggtcagccggacgactctgaaccggaagctcgtaccggtgctgt
tctgcgtcgtccgcgtcgtcacggtctggctaaacgtctgtaatactagagtcacacaggaaagtactagatgacagta
aagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaat
tattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaag
tgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagataga
atcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatc
atgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcga
agaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagtt
caattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattat
taacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagcttt
atcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaa
aggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagt
gctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagaacctgtaggatcgtacaggtttacttgtgagcggata
acaatatagtgtgtggaattgtgagcggataacaatttactagagacccaaatccaggaggtgaatctagtaggtggtt
aatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctactagagaaagaggagaaat
actagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcg
ttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactca
aatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgatt
atatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcg
ttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatat
aaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtatta
aagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctat
taatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatag
tgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata