BBa_K112711
1
BBa_K112711
rbs.spvR!
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of rbs.SpvR! basic part K112711
PCR of rbs.SpvR! al0027 and al0028 (933bp, EcoRI, BamHI, DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+933bp, L)
Product is pBca1256-K112711
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al0027 Forward Biobricking of rbs.spvR! CCATAGAATTCatgAGATCTAAAAAAGGAGATATTATGGATTTCTTG
al0028 Reverse Biobricking of rbs.spvR! CGttaGGATCCaggtcagaaggtggactg
</pre>
The spv repressor, with an rbs and stop codon.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
true
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112711_sequence
1
aaaaaaggagatattatggatttcttgattaataaaaaattaaaaattttcataacactgatggaaacaggttccttcagtatcgcaacatcagtactgtatatcacccgaaccccgctgagcagggttatttcagacctggaaagagagctgaaacaaagactctttatacggaagaatggcactcttatcccaaccgaatttgcacaaactatttatcgaaaagtaaaatcccattatattttcttacatgcactggagcaggaaatcggacctacgggtaaaacgaaacaactagaaataatatttgacgaaatttatccgggaagtttaaaaaatctgatcatttcagcactgaccatttccggccaaaaaacaaatataatggggagagccgttaacagccaaataatagaagaactgtgtcagacaaacaactgcattgttatttctgccagaaattattttcatcgggaatcgcttgtctgccggacatcagtggagggtggggtcatgttatttattcctaaaaaattctttctctgcggcaaacctgatatcaacaggctggccggaacacctgtactttttcatgagggggctaaaaattttaatctggacaccatataccatttttttgaacagacactaggtattaccaaccctgcattcagttttgataacgtcgatttgttcagttcactgtaccggttacaacaagggctggcgatgttactcatccccgtcagagtctgtcgggctctgggattatcaacagatcacgcactgcacatcaaaggcgtagcgctctgtacctccttgtattacccgaccaagaaacgggagacaccagatttatcgtaagctataaaactgatacagcagggaactgaaacagtccaccttctgacct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z