LOCUS       BBa_K112809             3003 bp    DNA     linear       22-Nov-2024
DEFINITION  T4 Lysis Device with Pbad
ACCESSION   BBa_K112809
VERSION     BBa_K112809.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     CDS             1235..1891
                     /label=T4 holin
     CDS             1219..1894
                     /label=BBa_K112805
     CDS             1919..2413
                     /label=T4 Endolysin
     CDS             1903..2416
                     /label=BBa_K112806
     stem_loop       2436..2479
                     /label=stem_loop
     misc_feature    2425..2504
                     /label=BBa_B0010
     terminator      2425..2504
                     /label=BBa_B0010
     stem_loop       2520..2539
                     /label=T7 TE
     misc_feature    2546..2546
                     /label=stop
     polyA_site      2540..2553
                     /label=polya
     misc_feature    2513..2553
                     /label=BBa_B0012
     terminator      2513..2553
                     /label=BBa_B0012
     promoter        2562..2596
                     /label=BBa_J23116
     CDS             2619..2912
                     /label=T4 antiholin
     CDS             2605..2915
                     /label=BBa_K112807
     stem_loop       2935..2978
                     /label=stem_loop
     misc_feature    2924..3003
                     /label=BBa_B0010
     terminator      2924..3003
                     /label=BBa_B0010
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagct taaaaggagg gtctatggca gcacctagaa tatcattttc
     1261 gccctctgat attctatttg gtgttctcga tcgcttgttc aaagataacg ctaccgggaa
     1321 ggttcttgct tcccgggtag ctgtcgtaat tcttttgttt ataatggcga ttgtttggta
     1381 taggggagat agtttctttg agtactataa gcaatcaaag tatgaaacat acagtgaaat
     1441 tattgaaaag gaaagaactg cacgctttga atctgtcgcc ctggaacaac tccagatagt
     1501 tcatatatca tctgaggcag actttagtgc ggtgtattct ttccgcccta aaaacttaaa
     1561 ctattttgtt gatattatag catacgaagg aaaattacct tcaacaataa gtgaaaaatc
     1621 acttggagga tatcctgttg ataaaactat ggatgaatat acagttcatt taaatggacg
     1681 tcattattat tccaactcaa aatttgcttt tttaccaact aaaaagccta ctcccgaaat
     1741 aaactacatg tacagttgtc catattttaa tttggataat atctatgctg gaacgataac
     1801 catgtactgg tatagaaatg atcatataag taatgaccgc cttgaatcaa tatgtgctca
     1861 ggcggccaga atattaggaa gggctaaata attatactag agatacttag gaggtattat
     1921 gaatatattt gaaatgttac gtatagatga aggtcttaga cttaaaatct ataaagacac
     1981 agaaggctat tacactattg gcatcggtca tttgcttaca aaaagtccat cacttaatgc
     2041 tgctaaatct gaattagata aagctattgg gcgtaattgc aatggtgtaa ttacaaaaga
     2101 tgaggctgaa aaactcttta atcaggatgt tgatgctgct gttcgcggaa tcctgagaaa
     2161 tgctaaatta aaaccggttt atgattctct tgatgcggtt cgtcgctgtg cattgattaa
     2221 tatggttttc caaatgggag aaaccggtgt ggcaggattt actaactctt tacgtatgct
     2281 tcaacaaaaa cgctgggatg aagcagcagt taacttagct aaaagtagat ggtataatca
     2341 aacacctaat cgcgcaaaac gagtcattac aacgtttaga actggcactt gggacgcgta
     2401 taaaaatcta taaagctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2461 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2521 gctcaccttc gggtgggcct ttctgcgttt atatactaga gttgacagct agctcagtcc
     2581 tagggactat gctagctact agaggatagg aggcctttat ggccttaaaa gcaacagcac
     2641 tttttgccat gctaggattg tcatttgttt tatctccatc gattgaagcg aatgtcgatc
     2701 ctcattttga taaatttatg gaatctggta ttaggcacgt ttatatgctt tttgaaaata
     2761 aaagcgtaga atcgtctgaa caattctata gttttatgag aacgacctat aaaaatgacc
     2821 cgtgctcttc tgattttgaa tgtatagagc gaggcgcgga gatggcacaa tcatacgcta
     2881 gaattatgaa cattaaattg gagactgaat gaaattacta gagccaggca tcaaataaaa
     2941 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     3001 ctc
//