BBa_K1129019
type: ComponentDefinition
persistent identity: BBa K1129019
display id: BBa_K1129019
version: 1
was generated by: iGEM to SBOL conversion
description: Complete caffeine synthesis pathway under pTET constitutive promoter
created: 2013-08-29T11:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: The constitutive promoter we used was from the 2012 Distribution Kit, part BBa_J23118. The caffeine biosynthesis genes we assembled were from the 2013 Distribution Kit and were submitted by the 2012 TU Munich team: parts BBa_K801070, BBa_K801071, BBa_K801072.
owned by: james
owned by: myers
mutable description: We combined the three caffeine synthesis genes in the Registry by standard assembly into a pSB1C3 vector. This vector had a constitutive promoter, BBa_J23118, and a bacterial consensus sequence cloned into it previously, so that the completed construct would allow constitutive expression of the entire caffeine biosynthesis pathway when the substrate xanthosine was present.
discontinued: false
dominant: false
experience: experience/None
group u list: _1441_
m user id: 0
owner id: 12783
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We have yet to test for expression, and we may find that codon optimization is needed.
star: false
creator: UBC iGEM 2013
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0015
component: BBa_K801072
component: BBa_B0034
component: BBa_J23118
component: BBa_K801071
component: BBa_K801070
sequence annotation: BBa_J23118
sequence annotation: BBa_B0034
sequence annotation: BBa_K801070
sequence annotation: BBa_B0015
sequence annotation: BBa_K801071
sequence annotation: BBa_K801072
sequence: https://synbiohub.org/public/igem/BBa_K1129019_sequence/1

BBa_B0015
type: Component
persistent identity: component2365730
display id: component2365730
version: 1
top level: BBa_K1129019
definition: BBa_B0015
access: public

BBa_K801072
type: Component
persistent identity: component2365723
display id: component2365723
version: 1
top level: BBa_K1129019
definition: BBa_K801072
access: public

BBa_B0034
type: Component
persistent identity: component2365717
display id: component2365717
version: 1
top level: BBa_K1129019
definition: BBa_B0034
access: public

BBa_J23118
type: Component
persistent identity: component2365715
display id: component2365715
version: 1
top level: BBa_K1129019
definition: BBa_J23118
access: public

BBa_B0015
type: Component
persistent identity: component2365714
display id: component2365714
version: 1
top level: BBa_K1129019
definition: BBa_B0015
access: public

BBa_K801071
type: Component
persistent identity: component2365707
display id: component2365707
version: 1
top level: BBa_K1129019
definition: BBa_K801071
access: public

BBa_B0034
type: Component
persistent identity: component2365701
display id: component2365701
version: 1
top level: BBa_K1129019
definition: BBa_B0034
access: public

BBa_J23118
type: Component
persistent identity: component2365699
display id: component2365699
version: 1
top level: BBa_K1129019
definition: BBa_J23118
access: public

BBa_B0015
type: Component
persistent identity: component2365698
display id: component2365698
version: 1
top level: BBa_K1129019
definition: BBa_B0015
access: public

BBa_K801070
type: Component
persistent identity: component2365691
display id: component2365691
version: 1
top level: BBa_K1129019
definition: BBa_K801070
access: public

BBa_B0034
type: Component
persistent identity: component2365685
display id: component2365685
version: 1
top level: BBa_K1129019
definition: BBa_B0034
access: public

BBa_J23118
type: Component
persistent identity: component2365683
display id: component2365683
version: 1
top level: BBa_K1129019
definition: BBa_J23118
access: public

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation2365683
display id: annotation2365683
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365683/range2365683/1
component: BBa_J23118

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type: Range
persistent identity: range2365683
display id: range2365683
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2365685
display id: annotation2365685
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365685/range2365685/1
component: BBa_B0034

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type: Range
persistent identity: range2365685
display id: range2365685
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K801070
type: SequenceAnnotation
persistent identity: annotation2365691
display id: annotation2365691
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365691/range2365691/1
component: BBa_K801070

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type: Range
persistent identity: range2365691
display id: range2365691
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 64
end: 1221
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2365698
display id: annotation2365698
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365698/range2365698/1
component: BBa_B0015

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type: Range
persistent identity: range2365698
display id: range2365698
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 1230
end: 1358
orientation: inline

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation2365699
display id: annotation2365699
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365699/range2365699/1
component: BBa_J23118

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type: Range
persistent identity: range2365699
display id: range2365699
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 1367
end: 1401
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2365701
display id: annotation2365701
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365701/range2365701/1
component: BBa_B0034

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type: Range
persistent identity: range2365701
display id: range2365701
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 1410
end: 1421
orientation: inline

BBa_K801071
type: SequenceAnnotation
persistent identity: annotation2365707
display id: annotation2365707
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365707/range2365707/1
component: BBa_K801071

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type: Range
persistent identity: range2365707
display id: range2365707
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 1430
end: 2605
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2365714
display id: annotation2365714
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365714/range2365714/1
component: BBa_B0015

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type: Range
persistent identity: range2365714
display id: range2365714
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 2614
end: 2742
orientation: inline

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation2365715
display id: annotation2365715
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365715/range2365715/1
component: BBa_J23118

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type: Range
persistent identity: range2365715
display id: range2365715
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 2751
end: 2785
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2365717
display id: annotation2365717
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365717/range2365717/1
component: BBa_B0034

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type: Range
persistent identity: range2365717
display id: range2365717
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 2794
end: 2805
orientation: inline

BBa_K801072
type: SequenceAnnotation
persistent identity: annotation2365723
display id: annotation2365723
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365723/range2365723/1
component: BBa_K801072

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type: Range
persistent identity: range2365723
display id: range2365723
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 2814
end: 4007
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2365730
display id: annotation2365730
version: 1
top level: BBa_K1129019
location: https://synbiohub.org/public/igem/BBa_K1129019/annotation2365730/range2365730/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1129019/annotation2365730/range2365730/1
type: Range
persistent identity: range2365730
display id: range2365730
version: 1
top level: BBa_K1129019
direction: direction/reverse
start: 4016
end: 4144
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23118
type: ComponentDefinition
persistent identity: BBa J23118
display id: BBa_J23118
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23118_sequence/1

BBa_K801070
type: ComponentDefinition
persistent identity: BBa K801070
display id: BBa_K801070
version: 1
was generated by: iGEM to SBOL conversion
description: xanthosine methyltransferase CaXMT1-strep
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB048793 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: xanthosine methyltransferase CaXMT1-strep from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Roman Prechtl
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Consensus sequence
sequence annotation: CaXMT1
sequence annotation: Start codon
sequence annotation: Strep Tag
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801070_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2198397
display id: annotation2198397
version: 1
top level: BBa_K801070
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1
type: Range
persistent identity: range2198397
display id: range2198397
version: 1
top level: BBa_K801070
direction: direction/forward
start: 1
end: 6
orientation: inline

CaXMT1
type: SequenceAnnotation
persistent identity: annotation2198398
display id: annotation2198398
version: 1
top level: BBa_K801070
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1
type: Range
persistent identity: range2198398
display id: range2198398
version: 1
top level: BBa_K801070
direction: direction/forward
start: 7
end: 1122
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198399
display id: annotation2198399
version: 1
top level: BBa_K801070
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2198399
display id: range2198399
version: 1
top level: BBa_K801070
direction: direction/forward
start: 7
end: 9
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198400
display id: annotation2198400
version: 1
top level: BBa_K801070
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1
type: Range
persistent identity: range2198400
display id: range2198400
version: 1
top level: BBa_K801070
direction: direction/forward
start: 1123
end: 1152
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198401
display id: annotation2198401
version: 1
top level: BBa_K801070
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1
type: Range
persistent identity: range2198401
display id: range2198401
version: 1
top level: BBa_K801070
direction: direction/forward
start: 1153
end: 1158
orientation: inline

BBa_K801071
type: ComponentDefinition
persistent identity: BBa K801071
display id: BBa_K801071
version: 1
was generated by: iGEM to SBOL conversion
description: 7-methylxanthine N-methyltransferase CaMXMT1-strep
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: Synthesized by IDT, Yeast codon optimized
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 7-methylxanthine N-methyltransferase CaMXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Roman Prechtl
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Consensus sequence
sequence annotation: CaMXMT1
sequence annotation: Start codon
sequence annotation: Strep Tag
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801071_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197876
display id: annotation2197876
version: 1
top level: BBa_K801071
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1
type: Range
persistent identity: range2197876
display id: range2197876
version: 1
top level: BBa_K801071
direction: direction/forward
start: 1
end: 6
orientation: inline

CaMXMT1
type: SequenceAnnotation
persistent identity: annotation2197709
display id: annotation2197709
version: 1
top level: BBa_K801071
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1
type: Range
persistent identity: range2197709
display id: range2197709
version: 1
top level: BBa_K801071
direction: direction/forward
start: 7
end: 1140
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198586
display id: annotation2198586
version: 1
top level: BBa_K801071
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1
type: Range
persistent identity: range2198586
display id: range2198586
version: 1
top level: BBa_K801071
direction: direction/forward
start: 7
end: 9
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198148
display id: annotation2198148
version: 1
top level: BBa_K801071
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1
type: Range
persistent identity: range2198148
display id: range2198148
version: 1
top level: BBa_K801071
direction: direction/forward
start: 1141
end: 1170
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198587
display id: annotation2198587
version: 1
top level: BBa_K801071
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1
type: Range
persistent identity: range2198587
display id: range2198587
version: 1
top level: BBa_K801071
direction: direction/forward
start: 1171
end: 1176
orientation: inline

BBa_K801072
type: ComponentDefinition
persistent identity: BBa K801072
display id: BBa_K801072
version: 1
was generated by: iGEM to SBOL conversion
description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1-strep
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB084125 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Roman Prechtl
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Consensus sequence
sequence annotation: Start codon
sequence annotation: CaDXMT1
sequence annotation: Strep Tag
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801072_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197893
display id: annotation2197893
version: 1
top level: BBa_K801072
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1
type: Range
persistent identity: range2197893
display id: range2197893
version: 1
top level: BBa_K801072
direction: direction/forward
start: 1
end: 6
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198600
display id: annotation2198600
version: 1
top level: BBa_K801072
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1
type: Range
persistent identity: range2198600
display id: range2198600
version: 1
top level: BBa_K801072
direction: direction/forward
start: 7
end: 9
orientation: inline

CaDXMT1
type: SequenceAnnotation
persistent identity: annotation2197894
display id: annotation2197894
version: 1
top level: BBa_K801072
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1
type: Range
persistent identity: range2197894
display id: range2197894
version: 1
top level: BBa_K801072
direction: direction/forward
start: 7
end: 1158
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198187
display id: annotation2198187
version: 1
top level: BBa_K801072
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1
type: Range
persistent identity: range2198187
display id: range2198187
version: 1
top level: BBa_K801072
direction: direction/forward
start: 1159
end: 1188
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198588
display id: annotation2198588
version: 1
top level: BBa_K801072
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1
type: Range
persistent identity: range2198588
display id: range2198588
version: 1
top level: BBa_K801072
direction: direction/forward
start: 1189
end: 1194
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1129019_sequence/1
type: Sequence
persistent identity: BBa K1129019 sequence
display id: BBa_K1129019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagagtacacaatgtctttacaagaagtcttgagaatgaacggtggtgaaggtgatacttcttacgctaaaaactccgcctacaatcaattggttttggctaaagttaagccagtcttggaacaatgcgtcagagaattattgagagctaacttgccaaacatcaacaagtgcattaaggttgctgatttgggttgtgcttctggtccaaatactttgttgactgttagagacatcgtccaatccattgataaggttggtcaagaaaagaagaacgaattggaaagaccaaccatccaaattttcttgaacgacttgttcccaaacgacttcaactctgtttttaagttgttgccatccttctacagaaagttggaaaaagaaaacggtagaaagatcggttcctgtttgattggtgctatgccaggttctttctactccagattatttcctgaagaatccatgcatttcttgcactcttgttattgcttgcaatggttgtctcaagttccatctggtttggttactgaattgggtatttctaccaacaagggttccatctactcttctaaagcttcaagattgccagttcaaaaggcctacttggatcaattcactaaggatttcaccacctttttgagaatccactccgaagaattattctcccacggtagaatgttgttgacctgtatatgtaagggtgttgaattggatgctagaaacgccattgatttgttggaaatggctatcaacgatttggttgttgaaggtcacttagaagaagaaaagttggactctttcaacttgccagtttacattccatctgccgaagaagttaagtgcatcgttgaagaagaaggttccttcgaaatcttgtacttggaaactttcaaggtcttgtacgatgccggtttctctattgatgatgaacatattaaggccgaatacgttgcctcttctgttagagctgtttacgaacctattttggcttctcatttcggtgaagccattatcccagatatctttcatagatttgctaagcacgctgctaaggttttgccattgggtaaaggtttttacaacaacttgatcatctccttggccaaaaagccagaaaagtctgatgtttctgcttggtcacatccacaattcgaaaagtgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagagtacacaatgtctttacaagaagtcttgcacatgaacgaaggtgaaggtgatacttcttacgctaagaatgcttcttacaacttggctttggctaaggttaagccattcttggaacaatgcatcagagaattattgagagccaacttgccaaacatcaacaagtgtattaaggttgctgatttgggttgtgcttctggtccaaatactttgttgactgttagagacatcgtccaatccattgataaggttggtcaagaagaaaagaacgaattggaaagaccaaccatccaaattttcttgaacgacttgttccaaaacgacttcaactccgtttttaagttgttgccatccttctacagaaagttggaaaaagaaaacggtagaaagatcggttcctgcttgatttctgctatgccaggttctttttacggtagattattccctgaagaatccatgcatttcttgcactcttgttactctgttcactggttgtctcaagttccatctggtttggttattgaattgggtattggtgctaacaagggttccatctattcttctaaaggttgta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23118_sequence/1
type: Sequence
persistent identity: BBa J23118 sequence
display id: BBa_J23118_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtattgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801070_sequence/1
type: Sequence
persistent identity: BBa K801070 sequence
display id: BBa_K801070_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacacaatgtctttacaagaagtcttgagaatgaacggtggtgaaggtgatacttcttacgctaaaaactccgcctacaatcaattggttttggctaaagttaagccagtcttggaacaatgcgtcagagaattattgagagctaacttgccaaacatcaacaagtgcattaaggttgctgatttgggttgtgcttctggtccaaatactttgttgactgttagagacatcgtccaatccattgataaggttggtcaagaaaagaagaacgaattggaaagaccaaccatccaaattttcttgaacgacttgttcccaaacgacttcaactctgtttttaagttgttgccatccttctacagaaagttggaaaaagaaaacggtagaaagatcggttcctgtttgattggtgctatgccaggttctttctactccagattatttcctgaagaatccatgcatttcttgcactcttgttattgcttgcaatggttgtctcaagttccatctggtttggttactgaattgggtatttctaccaacaagggttccatctactcttctaaagcttcaagattgccagttcaaaaggcctacttggatcaattcactaaggatttcaccacctttttgagaatccactccgaagaattattctcccacggtagaatgttgttgacctgtatatgtaagggtgttgaattggatgctagaaacgccattgatttgttggaaatggctatcaacgatttggttgttgaaggtcacttagaagaagaaaagttggactctttcaacttgccagtttacattccatctgccgaagaagttaagtgcatcgttgaagaagaaggttccttcgaaatcttgtacttggaaactttcaaggtcttgtacgatgccggtttctctattgatgatgaacatattaaggccgaatacgttgcctcttctgttagagctgtttacgaacctattttggcttctcatttcggtgaagccattatcccagatatctttcatagatttgctaagcacgctgctaaggttttgccattgggtaaaggtttttacaacaacttgatcatctccttggccaaaaagccagaaaagtctgatgtttctgcttggtcacatccacaattcgaaaagtgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801071_sequence/1
type: Sequence
persistent identity: BBa K801071 sequence
display id: BBa_K801071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacacaatgtctttacaagaagtcttgcacatgaacgaaggtgaaggtgatacttcttacgctaagaatgcttcttacaacttggctttggctaaggttaagccattcttggaacaatgcatcagagaattattgagagccaacttgccaaacatcaacaagtgtattaaggttgctgatttgggttgtgcttctggtccaaatactttgttgactgttagagacatcgtccaatccattgataaggttggtcaagaagaaaagaacgaattggaaagaccaaccatccaaattttcttgaacgacttgttccaaaacgacttcaactccgtttttaagttgttgccatccttctacagaaagttggaaaaagaaaacggtagaaagatcggttcctgcttgatttctgctatgccaggttctttttacggtagattattccctgaagaatccatgcatttcttgcactcttgttactctgttcactggttgtctcaagttccatctggtttggttattgaattgggtattggtgctaacaagggttccatctattcttctaaaggttgtagaccaccagttcaaaaggcttacttggatcaattcactaaggacttcaccactttcttgagaatccactccaaagaattattctccagaggtagaatgttgttgacctgtatctgtaaggttgacgaatttgatgaacctaacccattggatttgttggatatggccattaacgatttgatcgtcgaaggtttgttggaagaagaaaagttggactccttcaacattccattctttactccatctgccgaagaagttaagtgcatcgttgaagaagaaggttcttgcgaaatcttgtacttggaaactttcaaggctcattacgatgctgccttctctattgatgatgattacccagttagatcccacgaacaaatcaaagctgaatacgttgcctccttgatcagatctgtttacgaacctattttggcctctcatttcggtgaagctattatgccagatttgtttcacagattggctaaacatgctgctaaggttttacacatgggtaagggttgttacaacaacttgattatctccttggccaaaaagccagaaaagtctgatgtttctgcttggtcacatccacaattcgaaaagtgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801072_sequence/1
type: Sequence
persistent identity: BBa K801072 sequence
display id: BBa_K801072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacacaatgtctttacaagaagtcttgcatatgaacggtggtgaaggtgatacttcttacgctaagaactctttctacaacttgttcttgatcagagtcaagccaatcttggaacaatgcatccaagaattattgagagccaacttgccaaacatcaacaagtgtattaaggttgctgatttgggttgtgcttctggtccaaatactttgttgactgttagagacatcgtccaatccattgataaggttggtcaagaaaagaagaacgaattggaaagaccaaccatccaaattttcttgaacgacttgttccaaaacgacttcaactccgtttttaagtccttgccatccttctacagaaagttggaaaaagaaaacggtagaaagatcggttcctgtttgattggtgctatgccaggttctttttacggtagattattccctgaagaatccatgcatttcttgcattcttgttactgcttgcactggttgtctcaagttccatctggtttggttactgaattgggtatttctgctaacaagggttgcatctactcttctaaagcttcaagaccaccaattcaaaaggcctacttggatcaattcactaaggatttcaccactttcttgagaatccactccgaagaattgatcagtagaggtagaatgttgttgacctggatctgcaaagaagatgaatttgaaaacccaaactccatcgatttgttggaaatgtccatcaacgatttggttatcgaaggtcacttagaagaagaaaagttggactctttcaacgttccaatctatgctccatctaccgaagaagttaagtgcatcgttgaagaagaaggttccttcgaaatcttgtacttggaaacctttaaggttccatacgatgccggtttctctatcgatgatgattatcaaggtagatcccactctccagtttcttgtgatgaacatgctagagctgctcatgttgcttcagttgttagatctattttcgaacctatcgttgcctctcatttcggtgaagctattatgccagatttgtcccatagaattgctaagaatgctgccaaggttttgagatctggtaagggtttttacgactccttgattatttccttggccaaaaagccagaaaagtccgatgtttctgcttggtcacatccacaattcgaaaagtgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z