LOCUS BBa_K1129020 4429 bp DNA linear 04-May-2025 DEFINITION Complete caffeine synthesis pathway under arabinose-induced promoter ACCESSION BBa_K1129020 VERSION BBa_K1129020.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 misc_feature 159..164 /label=Consensus sequence CDS 165..1280 /label=CaXMT1 misc_feature 165..167 /label=Start codon misc_feature 1281..1310 /label=Strep Tag misc_feature 1311..1316 /label=Stop codon CDS 159..1316 /label=BBa_K801070 stem_loop 1336..1379 /label=stem_loop misc_feature 1325..1404 /label=BBa_B0010 terminator 1325..1404 /label=BBa_B0010 stem_loop 1420..1439 /label=T7 TE misc_feature 1446..1446 /label=stop polyA_site 1440..1453 /label=polya misc_feature 1413..1453 /label=BBa_B0012 terminator 1413..1453 /label=BBa_B0012 terminator 1325..1453 /label=BBa_B0015 promoter 1462..1591 /label=BBa_I13453 misc_feature 1604..1607 /label=conserved RBS 1600..1611 /label=BBa_B0034 CDS 1626..2759 /label=CaMXMT1 misc_feature 1620..1625 /label=Consensus sequence misc_feature 2760..2789 /label=Strep Tag misc_feature 1626..1628 /label=Start codon misc_feature 2790..2795 /label=Stop codon CDS 1620..2795 /label=BBa_K801071 stem_loop 2815..2858 /label=stem_loop misc_feature 2804..2883 /label=BBa_B0010 terminator 2804..2883 /label=BBa_B0010 stem_loop 2899..2918 /label=T7 TE misc_feature 2925..2925 /label=stop polyA_site 2919..2932 /label=polya misc_feature 2892..2932 /label=BBa_B0012 terminator 2892..2932 /label=BBa_B0012 terminator 2804..2932 /label=BBa_B0015 promoter 2941..3070 /label=BBa_I13453 misc_feature 3083..3086 /label=conserved RBS 3079..3090 /label=BBa_B0034 misc_feature 3099..3104 /label=Consensus sequence CDS 3105..4256 /label=CaDXMT1 misc_feature 4257..4286 /label=Strep Tag misc_feature 4287..4292 /label=Stop codon misc_feature 3105..3107 /label=Start codon CDS 3099..4292 /label=BBa_K801072 stem_loop 4312..4355 /label=stem_loop misc_feature 4301..4380 /label=BBa_B0010 terminator 4301..4380 /label=BBa_B0010 stem_loop 4396..4415 /label=T7 TE misc_feature 4422..4422 /label=stop polyA_site 4416..4429 /label=polya misc_feature 4389..4429 /label=BBa_B0012 terminator 4389..4429 /label=BBa_B0012 terminator 4301..4429 /label=BBa_B0015 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagagta cacaatgtct ttacaagaag 181 tcttgagaat gaacggtggt gaaggtgata cttcttacgc taaaaactcc gcctacaatc 241 aattggtttt ggctaaagtt aagccagtct tggaacaatg cgtcagagaa ttattgagag 301 ctaacttgcc aaacatcaac aagtgcatta aggttgctga tttgggttgt gcttctggtc 361 caaatacttt gttgactgtt agagacatcg tccaatccat tgataaggtt ggtcaagaaa 421 agaagaacga attggaaaga ccaaccatcc aaattttctt gaacgacttg ttcccaaacg 481 acttcaactc tgtttttaag ttgttgccat ccttctacag aaagttggaa aaagaaaacg 541 gtagaaagat cggttcctgt ttgattggtg ctatgccagg ttctttctac tccagattat 601 ttcctgaaga atccatgcat ttcttgcact cttgttattg cttgcaatgg ttgtctcaag 661 ttccatctgg tttggttact gaattgggta tttctaccaa caagggttcc atctactctt 721 ctaaagcttc aagattgcca gttcaaaagg cctacttgga tcaattcact aaggatttca 781 ccaccttttt gagaatccac tccgaagaat tattctccca cggtagaatg ttgttgacct 841 gtatatgtaa gggtgttgaa ttggatgcta gaaacgccat tgatttgttg gaaatggcta 901 tcaacgattt ggttgttgaa ggtcacttag aagaagaaaa gttggactct ttcaacttgc 961 cagtttacat tccatctgcc gaagaagtta agtgcatcgt tgaagaagaa ggttccttcg 1021 aaatcttgta cttggaaact ttcaaggtct tgtacgatgc cggtttctct attgatgatg 1081 aacatattaa ggccgaatac gttgcctctt ctgttagagc tgtttacgaa cctattttgg 1141 cttctcattt cggtgaagcc attatcccag atatctttca tagatttgct aagcacgctg 1201 ctaaggtttt gccattgggt aaaggttttt acaacaactt gatcatctcc ttggccaaaa 1261 agccagaaaa gtctgatgtt tctgcttggt cacatccaca attcgaaaag tgatgatact 1321 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1381 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1441 ttctgcgttt atatactaga gacattgatt atttgcacgg cgtcacactt tgctatgcca 1501 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta 1561 ctgtttctcc ataccgtttt tttgggctag ctactagaga aagaggagaa atactagagt 1621 acacaatgtc tttacaagaa gtcttgcaca tgaacgaagg tgaaggtgat acttcttacg 1681 ctaagaatgc ttcttacaac ttggctttgg ctaaggttaa gccattcttg gaacaatgca 1741 tcagagaatt attgagagcc aacttgccaa acatcaacaa gtgtattaag gttgctgatt 1801 tgggttgtgc ttctggtcca aatactttgt tgactgttag agacatcgtc caatccattg 1861 ataaggttgg tcaagaagaa aagaacgaat tggaaagacc aaccatccaa attttcttga 1921 acgacttgtt ccaaaacgac ttcaactccg tttttaagtt gttgccatcc ttctacagaa 1981 agttggaaaa agaaaacggt agaaagatcg gttcctgctt gatttctgct atgccaggtt 2041 ctttttacgg tagattattc cctgaagaat ccatgcattt cttgcactct tgttactctg 2101 ttcactggtt gtctcaagtt ccatctggtt tggttattga attgggtatt ggtgctaaca 2161 agggttccat ctattcttct aaaggttgta gaccaccagt tcaaaaggct tacttggatc 2221 aattcactaa ggacttcacc actttcttga gaatccactc caaagaatta ttctccagag 2281 gtagaatgtt gttgacctgt atctgtaagg ttgacgaatt tgatgaacct aacccattgg 2341 atttgttgga tatggccatt aacgatttga tcgtcgaagg tttgttggaa gaagaaaagt 2401 tggactcctt caacattcca ttctttactc catctgccga agaagttaag tgcatcgttg 2461 aagaagaagg ttcttgcgaa atcttgtact tggaaacttt caaggctcat tacgatgctg 2521 ccttctctat tgatgatgat tacccagtta gatcccacga acaaatcaaa gctgaatacg 2581 ttgcctcctt gatcagatct gtttacgaac ctattttggc ctctcatttc ggtgaagcta 2641 ttatgccaga tttgtttcac agattggcta aacatgctgc taaggtttta cacatgggta 2701 agggttgtta caacaacttg attatctcct tggccaaaaa gccagaaaag tctgatgttt 2761 ctgcttggtc acatccacaa ttcgaaaagt gatgatacta gagccaggca tcaaataaaa 2821 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 2881 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 2941 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 3001 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 3061 ttgggctagc tactagagaa agaggagaaa tactagagta cacaatgtct ttacaagaag 3121 tcttgcatat gaacggtggt gaaggtgata cttcttacgc taagaactct ttctacaact 3181 tgttcttgat cagagtcaag ccaatcttgg aacaatgcat ccaagaatta ttgagagcca 3241 acttgccaaa catcaacaag tgtattaagg ttgctgattt gggttgtgct tctggtccaa 3301 atactttgtt gactgttaga gacatcgtcc aatccattga taaggttggt caagaaaaga 3361 agaacgaatt ggaaagacca accatccaaa ttttcttgaa cgacttgttc caaaacgact 3421 tcaactccgt ttttaagtcc ttgccatcct tctacagaaa gttggaaaaa gaaaacggta 3481 gaaagatcgg ttcctgtttg attggtgcta tgccaggttc tttttacggt agattattcc 3541 ctgaagaatc catgcatttc ttgcattctt gttactgctt gcactggttg tctcaagttc 3601 catctggttt ggttactgaa ttgggtattt ctgctaacaa gggttgcatc tactcttcta 3661 aagcttcaag accaccaatt caaaaggcct acttggatca attcactaag gatttcacca 3721 ctttcttgag aatccactcc gaagaattga tcagtagagg tagaatgttg ttgacctgga 3781 tctgcaaaga agatgaattt gaaaacccaa actccatcga tttgttggaa atgtccatca 3841 acgatttggt tatcgaaggt cacttagaag aagaaaagtt ggactctttc aacgttccaa 3901 tctatgctcc atctaccgaa gaagttaagt gcatcgttga agaagaaggt tccttcgaaa 3961 tcttgtactt ggaaaccttt aaggttccat acgatgccgg tttctctatc gatgatgatt 4021 atcaaggtag atcccactct ccagtttctt gtgatgaaca tgctagagct gctcatgttg 4081 cttcagttgt tagatctatt ttcgaaccta tcgttgcctc tcatttcggt gaagctatta 4141 tgccagattt gtcccataga attgctaaga atgctgccaa ggttttgaga tctggtaagg 4201 gtttttacga ctccttgatt atttccttgg ccaaaaagcc agaaaagtcc gatgtttctg 4261 cttggtcaca tccacaattc gaaaagtgat gatactagag ccaggcatca aataaaacga 4321 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 4381 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //