BBa_K1129020
type: ComponentDefinition
persistent identity: BBa K1129020
display id: BBa_K1129020
version: 1
was generated by: iGEM to SBOL conversion
description: Complete caffeine synthesis pathway under arabinose-induced promoter
created: 2013-08-29T11:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: The caffeine biosynthesis genes we assembled were from the 2013 Distribution Kit and were submitted by the 2012 TU Munich team: parts BBa_K801070, BBa_K801071, BBa_K801072.
owned by: james
owned by: myers
mutable description: We combined the three caffeine synthesis genes in the Registry by standard assembly into a pSB1C3 vector. This vector had an arabinose-inducible promoter and a bacterial consensus sequence cloned into it previously, so that the completed construct would allow expression of the entire caffeine biosynthesis pathway when the substrate xanthosine and inducer arabinose was present.
discontinued: false
dominant: false
experience: experience/None
group u list: _1441_
m user id: 0
owner id: 12783
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We have yet to test for expression, and we may find that codon optimization is needed.
star: false
creator: UBC iGEM 2013
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_I13453
component: BBa_B0015
component: BBa_K801070
component: BBa_K801072
component: BBa_K801071
sequence annotation: BBa_I13453
sequence annotation: BBa_B0034
sequence annotation: BBa_K801070
sequence annotation: BBa_B0015
sequence annotation: BBa_K801071
sequence annotation: BBa_K801072
sequence: https://synbiohub.org/public/igem/BBa_K1129020_sequence/1

BBa_B0034
type: Component
persistent identity: component2365754
display id: component2365754
version: 1
top level: BBa_K1129020
definition: BBa_B0034
access: public

BBa_I13453
type: Component
persistent identity: component2365752
display id: component2365752
version: 1
top level: BBa_K1129020
definition: BBa_I13453
access: public

BBa_B0015
type: Component
persistent identity: component2365751
display id: component2365751
version: 1
top level: BBa_K1129020
definition: BBa_B0015
access: public

BBa_K801070
type: Component
persistent identity: component2365744
display id: component2365744
version: 1
top level: BBa_K1129020
definition: BBa_K801070
access: public

BBa_B0034
type: Component
persistent identity: component2365738
display id: component2365738
version: 1
top level: BBa_K1129020
definition: BBa_B0034
access: public

BBa_I13453
type: Component
persistent identity: component2365736
display id: component2365736
version: 1
top level: BBa_K1129020
definition: BBa_I13453
access: public

BBa_B0015
type: Component
persistent identity: component2365783
display id: component2365783
version: 1
top level: BBa_K1129020
definition: BBa_B0015
access: public

BBa_K801072
type: Component
persistent identity: component2365776
display id: component2365776
version: 1
top level: BBa_K1129020
definition: BBa_K801072
access: public

BBa_B0034
type: Component
persistent identity: component2365770
display id: component2365770
version: 1
top level: BBa_K1129020
definition: BBa_B0034
access: public

BBa_I13453
type: Component
persistent identity: component2365768
display id: component2365768
version: 1
top level: BBa_K1129020
definition: BBa_I13453
access: public

BBa_B0015
type: Component
persistent identity: component2365767
display id: component2365767
version: 1
top level: BBa_K1129020
definition: BBa_B0015
access: public

BBa_K801071
type: Component
persistent identity: component2365760
display id: component2365760
version: 1
top level: BBa_K1129020
definition: BBa_K801071
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2365736
display id: annotation2365736
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365736/range2365736/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365736/range2365736/1
type: Range
persistent identity: range2365736
display id: range2365736
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2365738
display id: annotation2365738
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365738/range2365738/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365738/range2365738/1
type: Range
persistent identity: range2365738
display id: range2365738
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 139
end: 150
orientation: inline

BBa_K801070
type: SequenceAnnotation
persistent identity: annotation2365744
display id: annotation2365744
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365744/range2365744/1
component: BBa_K801070

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365744/range2365744/1
type: Range
persistent identity: range2365744
display id: range2365744
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 159
end: 1316
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2365751
display id: annotation2365751
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365751/range2365751/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365751/range2365751/1
type: Range
persistent identity: range2365751
display id: range2365751
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 1325
end: 1453
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2365752
display id: annotation2365752
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365752/range2365752/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365752/range2365752/1
type: Range
persistent identity: range2365752
display id: range2365752
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 1462
end: 1591
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2365754
display id: annotation2365754
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365754/range2365754/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365754/range2365754/1
type: Range
persistent identity: range2365754
display id: range2365754
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 1600
end: 1611
orientation: inline

BBa_K801071
type: SequenceAnnotation
persistent identity: annotation2365760
display id: annotation2365760
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365760/range2365760/1
component: BBa_K801071

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365760/range2365760/1
type: Range
persistent identity: range2365760
display id: range2365760
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 1620
end: 2795
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2365767
display id: annotation2365767
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365767/range2365767/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365767/range2365767/1
type: Range
persistent identity: range2365767
display id: range2365767
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 2804
end: 2932
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2365768
display id: annotation2365768
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365768/range2365768/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365768/range2365768/1
type: Range
persistent identity: range2365768
display id: range2365768
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 2941
end: 3070
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2365770
display id: annotation2365770
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365770/range2365770/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365770/range2365770/1
type: Range
persistent identity: range2365770
display id: range2365770
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 3079
end: 3090
orientation: inline

BBa_K801072
type: SequenceAnnotation
persistent identity: annotation2365776
display id: annotation2365776
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365776/range2365776/1
component: BBa_K801072

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365776/range2365776/1
type: Range
persistent identity: range2365776
display id: range2365776
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 3099
end: 4292
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2365783
display id: annotation2365783
version: 1
top level: BBa_K1129020
location: https://synbiohub.org/public/igem/BBa_K1129020/annotation2365783/range2365783/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1129020/annotation2365783/range2365783/1
type: Range
persistent identity: range2365783
display id: range2365783
version: 1
top level: BBa_K1129020
direction: direction/reverse
start: 4301
end: 4429
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K801070
type: ComponentDefinition
persistent identity: BBa K801070
display id: BBa_K801070
version: 1
was generated by: iGEM to SBOL conversion
description: xanthosine methyltransferase CaXMT1-strep
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB048793 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: xanthosine methyltransferase CaXMT1-strep from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Roman Prechtl
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Consensus sequence
sequence annotation: CaXMT1
sequence annotation: Start codon
sequence annotation: Strep Tag
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801070_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2198397
display id: annotation2198397
version: 1
top level: BBa_K801070
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1
type: Range
persistent identity: range2198397
display id: range2198397
version: 1
top level: BBa_K801070
direction: direction/forward
start: 1
end: 6
orientation: inline

CaXMT1
type: SequenceAnnotation
persistent identity: annotation2198398
display id: annotation2198398
version: 1
top level: BBa_K801070
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1
type: Range
persistent identity: range2198398
display id: range2198398
version: 1
top level: BBa_K801070
direction: direction/forward
start: 7
end: 1122
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198399
display id: annotation2198399
version: 1
top level: BBa_K801070
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1
type: Range
persistent identity: range2198399
display id: range2198399
version: 1
top level: BBa_K801070
direction: direction/forward
start: 7
end: 9
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198400
display id: annotation2198400
version: 1
top level: BBa_K801070
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1
type: Range
persistent identity: range2198400
display id: range2198400
version: 1
top level: BBa_K801070
direction: direction/forward
start: 1123
end: 1152
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198401
display id: annotation2198401
version: 1
top level: BBa_K801070
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1
type: Range
persistent identity: range2198401
display id: range2198401
version: 1
top level: BBa_K801070
direction: direction/forward
start: 1153
end: 1158
orientation: inline

BBa_K801071
type: ComponentDefinition
persistent identity: BBa K801071
display id: BBa_K801071
version: 1
was generated by: iGEM to SBOL conversion
description: 7-methylxanthine N-methyltransferase CaMXMT1-strep
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: Synthesized by IDT, Yeast codon optimized
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 7-methylxanthine N-methyltransferase CaMXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Roman Prechtl
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Consensus sequence
sequence annotation: CaMXMT1
sequence annotation: Start codon
sequence annotation: Strep Tag
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801071_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197876
display id: annotation2197876
version: 1
top level: BBa_K801071
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1
type: Range
persistent identity: range2197876
display id: range2197876
version: 1
top level: BBa_K801071
direction: direction/forward
start: 1
end: 6
orientation: inline

CaMXMT1
type: SequenceAnnotation
persistent identity: annotation2197709
display id: annotation2197709
version: 1
top level: BBa_K801071
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1
type: Range
persistent identity: range2197709
display id: range2197709
version: 1
top level: BBa_K801071
direction: direction/forward
start: 7
end: 1140
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198586
display id: annotation2198586
version: 1
top level: BBa_K801071
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1
type: Range
persistent identity: range2198586
display id: range2198586
version: 1
top level: BBa_K801071
direction: direction/forward
start: 7
end: 9
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198148
display id: annotation2198148
version: 1
top level: BBa_K801071
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1
type: Range
persistent identity: range2198148
display id: range2198148
version: 1
top level: BBa_K801071
direction: direction/forward
start: 1141
end: 1170
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198587
display id: annotation2198587
version: 1
top level: BBa_K801071
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1
type: Range
persistent identity: range2198587
display id: range2198587
version: 1
top level: BBa_K801071
direction: direction/forward
start: 1171
end: 1176
orientation: inline

BBa_K801072
type: ComponentDefinition
persistent identity: BBa K801072
display id: BBa_K801072
version: 1
was generated by: iGEM to SBOL conversion
description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1-strep
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB084125 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Roman Prechtl
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Consensus sequence
sequence annotation: Start codon
sequence annotation: CaDXMT1
sequence annotation: Strep Tag
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801072_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197893
display id: annotation2197893
version: 1
top level: BBa_K801072
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1
type: Range
persistent identity: range2197893
display id: range2197893
version: 1
top level: BBa_K801072
direction: direction/forward
start: 1
end: 6
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198600
display id: annotation2198600
version: 1
top level: BBa_K801072
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1
type: Range
persistent identity: range2198600
display id: range2198600
version: 1
top level: BBa_K801072
direction: direction/forward
start: 7
end: 9
orientation: inline

CaDXMT1
type: SequenceAnnotation
persistent identity: annotation2197894
display id: annotation2197894
version: 1
top level: BBa_K801072
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1
type: Range
persistent identity: range2197894
display id: range2197894
version: 1
top level: BBa_K801072
direction: direction/forward
start: 7
end: 1158
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198187
display id: annotation2198187
version: 1
top level: BBa_K801072
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1
type: Range
persistent identity: range2198187
display id: range2198187
version: 1
top level: BBa_K801072
direction: direction/forward
start: 1159
end: 1188
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198588
display id: annotation2198588
version: 1
top level: BBa_K801072
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1
type: Range
persistent identity: range2198588
display id: range2198588
version: 1
top level: BBa_K801072
direction: direction/forward
start: 1189
end: 1194
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1129020_sequence/1
type: Sequence
persistent identity: BBa K1129020 sequence
display id: BBa_K1129020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagagtacacaatgtctttacaagaagtcttgagaatgaacggtggtgaaggtgatacttcttacgctaaaaactccgcctacaatcaattggttttggctaaagttaagccagtcttggaacaatgcgtcagagaattattgagagctaacttgccaaacatcaacaagtgcattaaggttgctgatttgggttgtgcttctggtccaaatactttgttgactgttagagacatcgtccaatccattgataaggttggtcaagaaaagaagaacgaattggaaagaccaaccatccaaattttcttgaacgacttgttcccaaacgacttcaactctgtttttaagttgttgccatccttctacagaaagttggaaaaagaaaacggtagaaagatcggttcctgtttgattggtgctatgccaggttctttctactccagattatttcctgaagaatccatgcatttcttgcactcttgttattgcttgcaatggttgtctcaagttccatctggtttggttactgaattgggtatttctaccaacaagggttccatctactcttctaaagcttcaagattgccagttcaaaaggcctacttggatcaattcactaaggatttcaccacctttttgagaatccactccgaagaattattctcccacggtagaatgttgttgacctgtatatgtaagggtgttgaattggatgctagaaacgccattgatttgttggaaatggctatcaacgatttggttgttgaaggtcacttagaagaagaaaagttggactctttcaacttgccagtttacattccatctgccgaagaagttaagtgcatcgttgaagaagaaggttccttcgaaatcttgtacttggaaactttcaaggtcttgtacgatgccggtttctctattgatgatgaacatattaaggccgaatacgttgcctcttctgttagagctgtttacgaacctattttggcttctcatttcggtgaagccattatcccagatatctttcatagatttgctaagcacgctgctaaggttttgccattgggtaaaggtttttacaacaacttgatcatctccttggccaaaaagccagaaaagtctgatgtttctgcttggtcacatccacaattcgaaaagtgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagagtacacaatgtctttacaagaagtcttgcacatgaacgaaggtgaaggtgatacttcttacgctaagaatgcttcttacaacttggctttggctaaggttaagccattcttggaacaatgcatcagagaattattgagagccaacttgccaaacatcaacaagtgtattaaggttgctgatttgggttgtgcttctggtccaaatactttgttgactgttagagacatcgtccaatccattgataaggttggtcaagaagaaaagaacgaattggaaagaccaaccatccaaattttcttgaacgacttgttccaaaacgacttcaactccgtttttaagttgttgccatccttctacagaaagttggaaaaagaaaacggt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801070_sequence/1
type: Sequence
persistent identity: BBa K801070 sequence
display id: BBa_K801070_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacacaatgtctttacaagaagtcttgagaatgaacggtggtgaaggtgatacttcttacgctaaaaactccgcctacaatcaattggttttggctaaagttaagccagtcttggaacaatgcgtcagagaattattgagagctaacttgccaaacatcaacaagtgcattaaggttgctgatttgggttgtgcttctggtccaaatactttgttgactgttagagacatcgtccaatccattgataaggttggtcaagaaaagaagaacgaattggaaagaccaaccatccaaattttcttgaacgacttgttcccaaacgacttcaactctgtttttaagttgttgccatccttctacagaaagttggaaaaagaaaacggtagaaagatcggttcctgtttgattggtgctatgccaggttctttctactccagattatttcctgaagaatccatgcatttcttgcactcttgttattgcttgcaatggttgtctcaagttccatctggtttggttactgaattgggtatttctaccaacaagggttccatctactcttctaaagcttcaagattgccagttcaaaaggcctacttggatcaattcactaaggatttcaccacctttttgagaatccactccgaagaattattctcccacggtagaatgttgttgacctgtatatgtaagggtgttgaattggatgctagaaacgccattgatttgttggaaatggctatcaacgatttggttgttgaaggtcacttagaagaagaaaagttggactctttcaacttgccagtttacattccatctgccgaagaagttaagtgcatcgttgaagaagaaggttccttcgaaatcttgtacttggaaactttcaaggtcttgtacgatgccggtttctctattgatgatgaacatattaaggccgaatacgttgcctcttctgttagagctgtttacgaacctattttggcttctcatttcggtgaagccattatcccagatatctttcatagatttgctaagcacgctgctaaggttttgccattgggtaaaggtttttacaacaacttgatcatctccttggccaaaaagccagaaaagtctgatgtttctgcttggtcacatccacaattcgaaaagtgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801071_sequence/1
type: Sequence
persistent identity: BBa K801071 sequence
display id: BBa_K801071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacacaatgtctttacaagaagtcttgcacatgaacgaaggtgaaggtgatacttcttacgctaagaatgcttcttacaacttggctttggctaaggttaagccattcttggaacaatgcatcagagaattattgagagccaacttgccaaacatcaacaagtgtattaaggttgctgatttgggttgtgcttctggtccaaatactttgttgactgttagagacatcgtccaatccattgataaggttggtcaagaagaaaagaacgaattggaaagaccaaccatccaaattttcttgaacgacttgttccaaaacgacttcaactccgtttttaagttgttgccatccttctacagaaagttggaaaaagaaaacggtagaaagatcggttcctgcttgatttctgctatgccaggttctttttacggtagattattccctgaagaatccatgcatttcttgcactcttgttactctgttcactggttgtctcaagttccatctggtttggttattgaattgggtattggtgctaacaagggttccatctattcttctaaaggttgtagaccaccagttcaaaaggcttacttggatcaattcactaaggacttcaccactttcttgagaatccactccaaagaattattctccagaggtagaatgttgttgacctgtatctgtaaggttgacgaatttgatgaacctaacccattggatttgttggatatggccattaacgatttgatcgtcgaaggtttgttggaagaagaaaagttggactccttcaacattccattctttactccatctgccgaagaagttaagtgcatcgttgaagaagaaggttcttgcgaaatcttgtacttggaaactttcaaggctcattacgatgctgccttctctattgatgatgattacccagttagatcccacgaacaaatcaaagctgaatacgttgcctccttgatcagatctgtttacgaacctattttggcctctcatttcggtgaagctattatgccagatttgtttcacagattggctaaacatgctgctaaggttttacacatgggtaagggttgttacaacaacttgattatctccttggccaaaaagccagaaaagtctgatgtttctgcttggtcacatccacaattcgaaaagtgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801072_sequence/1
type: Sequence
persistent identity: BBa K801072 sequence
display id: BBa_K801072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacacaatgtctttacaagaagtcttgcatatgaacggtggtgaaggtgatacttcttacgctaagaactctttctacaacttgttcttgatcagagtcaagccaatcttggaacaatgcatccaagaattattgagagccaacttgccaaacatcaacaagtgtattaaggttgctgatttgggttgtgcttctggtccaaatactttgttgactgttagagacatcgtccaatccattgataaggttggtcaagaaaagaagaacgaattggaaagaccaaccatccaaattttcttgaacgacttgttccaaaacgacttcaactccgtttttaagtccttgccatccttctacagaaagttggaaaaagaaaacggtagaaagatcggttcctgtttgattggtgctatgccaggttctttttacggtagattattccctgaagaatccatgcatttcttgcattcttgttactgcttgcactggttgtctcaagttccatctggtttggttactgaattgggtatttctgctaacaagggttgcatctactcttctaaagcttcaagaccaccaattcaaaaggcctacttggatcaattcactaaggatttcaccactttcttgagaatccactccgaagaattgatcagtagaggtagaatgttgttgacctggatctgcaaagaagatgaatttgaaaacccaaactccatcgatttgttggaaatgtccatcaacgatttggttatcgaaggtcacttagaagaagaaaagttggactctttcaacgttccaatctatgctccatctaccgaagaagttaagtgcatcgttgaagaagaaggttccttcgaaatcttgtacttggaaacctttaaggttccatacgatgccggtttctctatcgatgatgattatcaaggtagatcccactctccagtttcttgtgatgaacatgctagagctgctcatgttgcttcagttgttagatctattttcgaacctatcgttgcctctcatttcggtgaagctattatgccagatttgtcccatagaattgctaagaatgctgccaaggttttgagatctggtaagggtttttacgactccttgattatttccttggccaaaaagccagaaaagtccgatgtttctgcttggtcacatccacaattcgaaaagtgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z