LOCUS       BBa_K1129022            1029 bp    DNA     linear       25-Nov-2024
DEFINITION  Enoyl-CoA hydratase/aldolase (Ech) under pTET constitutive promoter
ACCESSION   BBa_K1129022
VERSION     BBa_K1129022.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..892
                     /label=BBa_K1129002
     stem_loop       912..955
                     /label=stem_loop
     misc_feature    901..980
                     /label=BBa_B0010
     terminator      901..980
                     /label=BBa_B0010
     stem_loop       996..1015
                     /label=T7 TE
     misc_feature    1022..1022
                     /label=stop
     polyA_site      1016..1029
                     /label=polya
     misc_feature    989..1029
                     /label=BBa_B0012
     terminator      989..1029
                     /label=BBa_B0012
     terminator      901..1029
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatgagcaaa tacgaaggcc gctggaccac cgtgaaggtc gaactggaag cgggcatcgc
      121 ctgggtgacc ctcaatcgcc cggaaaaacg caatgccatg agccccaccc tgaaccggga
      181 aatggtcgac gtgctggaaa cccttgagca ggacgctgac gctggcgtgc tggtattgac
      241 cggtgccggc gagtcctgga ccgccggcat ggacctgaag gagtacttcc gcgaggtgga
      301 cgccggcccg gaaatcctcc aggaaaagat tcgtcgcgaa gcctcgcaat ggcaatggaa
      361 gttgctgcgt ctgtatgcca aaccgaccat cgccatggtc aacggctggt gcttcggcgg
      421 cggcttcagc ccactggtgg catgcgacct ggcgatctgc gccaacgaag cgaccttcgg
      481 cctgtcggaa atcaactggg gcatcccgcc tggtaacctg gtcagcaagg ccatggccga
      541 taccgttggc catcgtcagt cgctgtacta catcatgacc ggcaagacct tcgatggtcg
      601 caaggctgcc gagatgggcc tggtgaacga cagtgtgccg ctggccgagc tgcgtgaaac
      661 cacccgcgag ttggcgctga acctgctgga aaagaacccg gtggtgctgc gtgccgcgaa
      721 gaatggcttc aagcgttgcc gcgagctgac ctgggaacag aacgaggact acctctacgc
      781 caagctcgac cagtcgcgcc tgctggacac taccggcggc cgcgagcagg gcatgaagca
      841 gttcctcgac gacaagagca tcaagccagg ccttcaggcc tacaagcgct gatactagag
      901 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      961 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1021 gcgtttata
//