BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_I13453 1 BBa_I13453 Pbad promoter 2005-05-24T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PBad promoter from I0500 without AraC. false false _11_ 0 253 6 In stock false true jkm BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1129023 1 BBa_K1129023 Feruloyl-CoA-Synthetase (FCS) under arabinose promoter 2013-09-15T11:00:00Z 2015-05-08T01:09:19Z Arab+rbs+fcs+term Arab+rbs+fcs+term false false _1441_ 0 9774 9 In stock true None false UBC iGEM 2013 component2353622 1 BBa_B0015 component2353614 1 BBa_B0034 component2353615 1 BBa_K1129001 component2353612 1 BBa_I13453 annotation2353622 1 BBa_B0015 range2353622 1 1935 2063 annotation2353614 1 BBa_B0034 range2353614 1 139 150 annotation2353612 1 BBa_I13453 range2353612 1 1 130 annotation2353615 1 BBa_K1129001 range2353615 1 157 1926 BBa_K1129001 1 BBa_K1129001 Feruloyl-CoA-Synthetase (FCS) from Pseudomonas putida KT2440 2013-08-29T11:00:00Z 2015-05-08T01:09:19Z Pseudomonas putida KT2440 This is a feruloyl-CoA-synthetase (fcs) from Pseudomonas putida KT2440 which converts ferrulic acid to feruloyl CoA. false false _1441_ 0 9774 9 It's complicated true Illegal cut sites removed false UBC iGEM 2013 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I13453_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1129023_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgcaacctgtcgagccactggcgccgctgccggcgcgcctgctcgagcgcctggtgcattgggcccaggtgcgcccggacaccactttcatcgcggcacgccaggcagacggtgcctggcgttcgatcagctacgtgcagatgctcgccgatgtgcgcaccatcgccgccaacttgctaggactgggcctcagtgccgagcgcccgctggcgctgctttccggcaacgacatcgaacacctgcaaatcgccctcggcgccatgtatgccggtattgcctattgcccggtgtcgccggcctacgcgctgttgtcgcaagacttcgccaagttgcgccatgtctgcgaggtgctcacccccggagtggtcttcgtcagcgacagccagccgttccagcgcgccttcgaggcggtgctggacgattcggtcggcgtgatcagcgtgcgtggccaggtcgcaggtcgcccccatataagcttcgacagcctgttgcaaccgggtgacctggcggcggccgatgcggctttcgccgccaccgggccggacaccatcgccaaattcctcttcacctcgggctcgaccaagctgcccaaggcggtgatcaccacccagcgcatgctgtgcgccaatcagcagatgcttcttcagacttttccgacgttcgccgaggagccgccggtgctggtggactggctgccgtggaaccacacgttcggcggtagccacaacctcggcatcgtgctttacaacgggggcagtttctacctggacgccggcaagccgaccccgcaaggcttcgccgagaccttgcgcaatctgcgcgagatttcccccacggcctacctcaccgtacccaagggctgggaggaactggtcaaggcactggagcaggaccccgcgctacgcgaggtgttctttgcccgcatcaagctgttcttctttgccgccgcaggcctgtcgcaaagcgtctgggaccggctggaccgcattgccgagcaacactgtggcgaacgcatccgcatgatggccggccttggcatgaccgaagcctcgccatcgtgcaccttcaccaccgggcctttgtcgatggccggctatgtcgggctgccggcacctggctgcgaagtgaagctggtgccggtgggcgacaagctcgaggcgcgcttccgtggcccgcatatcatgccgggctactggcgctcgccgcagcagaccgccgaggcgttcgacgaggagggcttctactgttcgggcgacgcgttgaagctggccgatgccaggcagcccgagcttggcctgatgttcgatggccgtatcgctgaggacttcaaactttcgtccggggtattcgtcagtgtcgggccgctgcgcaaccgcgcagtgctggagggctcgccttacgtacaggacatcgtggtcaccgcgccggaccgtgaatgcctgggcctgctggtgttcccgcgtctgcccgagtgtcggcgcctggccgggctggcagaggatgccagcgatgcgcgggtgctggccaacgacaccgtgcgcagttggttcgctgactggctggagcgcttgaaccgcgatgcccaaggcaacgccagccgtatcgaatggctgtcgctgctggccgagccgccgtcgatcgacgccggtgaaatcaccgacaagggctcgatcaatcagcgcgccgtgcttcagcggcgcgccgctcaggtcgaggcgctgtaccgtggcgaagaccccgacgcattgcacgccaaggtgcggccttgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1129001_sequence 1 atgcaacctgtcgagccactggcgccgctgccggcgcgcctgctcgagcgcctggtgcattgggcccaggtgcgcccggacaccactttcatcgcggcacgccaggcagacggtgcctggcgttcgatcagctacgtgcagatgctcgccgatgtgcgcaccatcgccgccaacttgctaggactgggcctcagtgccgagcgcccgctggcgctgctttccggcaacgacatcgaacacctgcaaatcgccctcggcgccatgtatgccggtattgcctattgcccggtgtcgccggcctacgcgctgttgtcgcaagacttcgccaagttgcgccatgtctgcgaggtgctcacccccggagtggtcttcgtcagcgacagccagccgttccagcgcgccttcgaggcggtgctggacgattcggtcggcgtgatcagcgtgcgtggccaggtcgcaggtcgcccccatataagcttcgacagcctgttgcaaccgggtgacctggcggcggccgatgcggctttcgccgccaccgggccggacaccatcgccaaattcctcttcacctcgggctcgaccaagctgcccaaggcggtgatcaccacccagcgcatgctgtgcgccaatcagcagatgcttcttcagacttttccgacgttcgccgaggagccgccggtgctggtggactggctgccgtggaaccacacgttcggcggtagccacaacctcggcatcgtgctttacaacgggggcagtttctacctggacgccggcaagccgaccccgcaaggcttcgccgagaccttgcgcaatctgcgcgagatttcccccacggcctacctcaccgtacccaagggctgggaggaactggtcaaggcactggagcaggaccccgcgctacgcgaggtgttctttgcccgcatcaagctgttcttctttgccgccgcaggcctgtcgcaaagcgtctgggaccggctggaccgcattgccgagcaacactgtggcgaacgcatccgcatgatggccggccttggcatgaccgaagcctcgccatcgtgcaccttcaccaccgggcctttgtcgatggccggctatgtcgggctgccggcacctggctgcgaagtgaagctggtgccggtgggcgacaagctcgaggcgcgcttccgtggcccgcatatcatgccgggctactggcgctcgccgcagcagaccgccgaggcgttcgacgaggagggcttctactgttcgggcgacgcgttgaagctggccgatgccaggcagcccgagcttggcctgatgttcgatggccgtatcgctgaggacttcaaactttcgtccggggtattcgtcagtgtcgggccgctgcgcaaccgcgcagtgctggagggctcgccttacgtacaggacatcgtggtcaccgcgccggaccgtgaatgcctgggcctgctggtgttcccgcgtctgcccgagtgtcggcgcctggccgggctggcagaggatgccagcgatgcgcgggtgctggccaacgacaccgtgcgcagttggttcgctgactggctggagcgcttgaaccgcgatgcccaaggcaacgccagccgtatcgaatggctgtcgctgctggccgagccgccgtcgatcgacgccggtgaaatcaccgacaagggctcgatcaatcagcgcgccgtgcttcagcggcgcgccgctcaggtcgaggcgctgtaccgtggcgaagaccccgacgcattgcacgccaaggtgcggccttga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z