BBa_K1129026 1 BBa_K1129026 Phenylalanine Ammonia Lyase (Enc-P) under pTET constitutive promoter 2013-09-15T11:00:00Z 2015-05-08T01:09:19Z Const+rbs+EncP+term Const+rbs+EncP+term false false _1441_ 0 9774 9 It's complicated true None false UBC iGEM 2013 component2353677 1 BBa_B0015 component2353670 1 BBa_K1129003 component2353667 1 BBa_J23118 component2353669 1 BBa_B0034 annotation2353667 1 BBa_J23118 range2353667 1 1 35 annotation2353677 1 BBa_B0015 range2353677 1 1642 1770 annotation2353669 1 BBa_B0034 range2353669 1 44 55 annotation2353670 1 BBa_K1129003 range2353670 1 62 1633 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1129003 1 BBa_K1129003 Phenylalanine Ammonia Lyase (Enc-P) from Streptomyces maritimus 2013-08-29T11:00:00Z 2015-05-08T01:09:19Z Streptomyces maritimus This is a phenylalanine ammonia lyase (PAL) from Streptomyces maritimus which converts amino acid phenylalanine into cinnamic acid. false false _1441_ 0 9774 9 In stock true Illegal cut sites removed false UBC iGEM 2013 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J23118 1 BBa_J23118 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1129003_sequence 1 atgaccttcgtcatagagctcgacatgaacgtcacgctcgaccaacttgaggacgcggcgcgacagcgcacgcccgtggagctgtccgcacccgtccgctcccgcgtccgcgcctcgcgcgacgtgttggtgaagttcgtgcaggacgaacgtgtcatctacggggtcaacaccagcatggggggcttcgtcgaccacctcgtcccggtgtcccaggcccggcagctccaggagaacctgatcaacgcggtcgccaccaacgtgggggcgtatctggacgacacgaccgcccggaccatcatgctgtcccgcatcgtgtcgctggcgcgcgggaactccgcgatcaccccggcgaatctggacaagctggtggccgtactcaacgccgggatcgtgccgtgcatcccggagaagggctctttgggcaccagcggtgacctcggcccgctggccgcgatcgccctggtgtgcgcggggcagtggaaggcccgctacaacggtcagatcatgcccgggcggcaggccctgtccgaggccggcgtcgagccgatggagctgagctacaaggatggcctggccctgatcaacggcacgtcaggcatggtcggcctgggcaccatggtcctccaggccgcgcgccggctcgtggaccgctaccttcaggtgtccgcgttgtcggtcgagggcctggcaggcatgacgaaaccgttcgaccctcgcgtgcacggcgtcaagccgcaccgcgggcagcgtcaggtggcctcgcggttgtgggaggggcttgccgactcgcacctggcggtcaacgaactggacaccgagcagaccctggccggagagatgggcacggtcgccaaggccggttcgctggcgatcgaggacgcctactccatccggtgcacgccgcagatcctcggtcccgtggtcgatgtgctggaccggatcggggcgacccttcaggacgagctgaactcctccaacgacaacccgatcgtcctgccggaggaggcggaggtgttccacaacgggcacttccacggccagtacgtggccatggccatggaccacctgaacatggccctggccaccgtgaccaatctcgccaaccggcgcgtggaccgcttcctggacaagagcaacagcaacgggctgcccgccttcctgtgccgggaagatccgggactgcgcctgggcctgatgggcggccagttcatgaccgcgtcgatcaccgcggagacccgcaccctgaccattccgatgtcggtgcagtccctcacgagtacggcggacttccaggacatcgtgtccttcggattcgtcgccgcccgccgcgcccgggaggtactcaccaacgctgcctacgtggtggccttcgagctgctgtgcgcctgccaggccgtcgacatccgcggcgcggacaaactgtcctccttcacccgcccgctctatgagcgcacccgcaagatcgtgccgttcttcgaccgggacgagaccatcaccgactacgtcgagaagctggcggccgacctgatcgcgggcgagcccgtcgacgctgccgtggcggcgcactga BBa_J23118_sequence 1 ttgacggctagctcagtcctaggtattgtgctagc BBa_K1129026_sequence 1 ttgacggctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgaccttcgtcatagagctcgacatgaacgtcacgctcgaccaacttgaggacgcggcgcgacagcgcacgcccgtggagctgtccgcacccgtccgctcccgcgtccgcgcctcgcgcgacgtgttggtgaagttcgtgcaggacgaacgtgtcatctacggggtcaacaccagcatggggggcttcgtcgaccacctcgtcccggtgtcccaggcccggcagctccaggagaacctgatcaacgcggtcgccaccaacgtgggggcgtatctggacgacacgaccgcccggaccatcatgctgtcccgcatcgtgtcgctggcgcgcgggaactccgcgatcaccccggcgaatctggacaagctggtggccgtactcaacgccgggatcgtgccgtgcatcccggagaagggctctttgggcaccagcggtgacctcggcccgctggccgcgatcgccctggtgtgcgcggggcagtggaaggcccgctacaacggtcagatcatgcccgggcggcaggccctgtccgaggccggcgtcgagccgatggagctgagctacaaggatggcctggccctgatcaacggcacgtcaggcatggtcggcctgggcaccatggtcctccaggccgcgcgccggctcgtggaccgctaccttcaggtgtccgcgttgtcggtcgagggcctggcaggcatgacgaaaccgttcgaccctcgcgtgcacggcgtcaagccgcaccgcgggcagcgtcaggtggcctcgcggttgtgggaggggcttgccgactcgcacctggcggtcaacgaactggacaccgagcagaccctggccggagagatgggcacggtcgccaaggccggttcgctggcgatcgaggacgcctactccatccggtgcacgccgcagatcctcggtcccgtggtcgatgtgctggaccggatcggggcgacccttcaggacgagctgaactcctccaacgacaacccgatcgtcctgccggaggaggcggaggtgttccacaacgggcacttccacggccagtacgtggccatggccatggaccacctgaacatggccctggccaccgtgaccaatctcgccaaccggcgcgtggaccgcttcctggacaagagcaacagcaacgggctgcccgccttcctgtgccgggaagatccgggactgcgcctgggcctgatgggcggccagttcatgaccgcgtcgatcaccgcggagacccgcaccctgaccattccgatgtcggtgcagtccctcacgagtacggcggacttccaggacatcgtgtccttcggattcgtcgccgcccgccgcgcccgggaggtactcaccaacgctgcctacgtggtggccttcgagctgctgtgcgcctgccaggccgtcgacatccgcggcgcggacaaactgtcctccttcacccgcccgctctatgagcgcacccgcaagatcgtgccgttcttcgaccgggacgagaccatcaccgactacgtcgagaagctggcggccgacctgatcgcgggcgagcccgtcgacgctgccgtggcggcgcactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z