LOCUS       BBa_K1129030            2441 bp    DNA     linear       26-Nov-2024
DEFINITION  Phenylalanine ammonia lyase under arabinose promoter
ACCESSION   BBa_K1129030
VERSION     BBa_K1129030.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..159
                     /label=Start codon
     misc_feature    1512..1512
                     /label=Mutation
     CDS             157..2304
                     /label=PAL
     misc_feature    1514..1516
                     /label=Deletion of Cys
     misc_feature    1520..1520
                     /label=Phe instead of Ser
     CDS             157..2304
                     /label=BBa_K801091
     stem_loop       2324..2367
                     /label=stem_loop
     misc_feature    2313..2392
                     /label=BBa_B0010
     terminator      2313..2392
                     /label=BBa_B0010
     stem_loop       2408..2427
                     /label=T7 TE
     misc_feature    2434..2434
                     /label=stop
     polyA_site      2428..2441
                     /label=polya
     misc_feature    2401..2441
                     /label=BBa_B0012
     terminator      2401..2441
                     /label=BBa_B0012
     terminator      2313..2441
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg caccctcgct cgactcgatc
      181 tcgcactcgt tcgcaaacgg cgtcgcatcc gcaaagcagg ctgtcaatgg cgcctcgacc
      241 aacctcgcag tcgcaggctc gcacctgccc acaacccagg tcacgcaggt cgacatcgtc
      301 gagaagatgc tcgccgcgcc gaccgactcg acgctcgaac tcgacggcta ctcgctcaac
      361 ctcggagacg tcgtctcggc cgcgaggaag ggcaggcctg tccgcgtcaa ggacagcgac
      421 gagatccgct caaagattga caaatcggtc gagttcttgc gctcgcaact ctccatgagc
      481 gtctacggcg ccacgactgg atttggcgga tccgcagaca cccgcaccga ggacgccatc
      541 tcgctccaga aggctctcct cgagcaccag ctctgcggtg ttctcccttc gtcgttcgac
      601 tcgttccgcc tcggccgcgg tctcgagaac tcgcttcccc tcgaggttgt tcgcggcgcc
      661 atgacaatcc gcgtcaacag cttgacccgc ggccactcgg ctgtccgcct cgtcgtcctc
      721 gaggcgctca ccaacttcct caaccacggc atcaccccca tcgtccccct ccgcggcacc
      781 atctctgcgt cgggcgacct ctctcctctc tcctacattg cagcggccat cagcggtcac
      841 ccggacagca aggtgcacgt cgtccacgag ggcaaggaga agatcctgta cgcccgcgag
      901 gcgatggcgc tcttcaacct cgagcccgtc gtcctcggcc cgaaggaagg tctcggtctc
      961 gtcaacggca ccgccgtctc agcatcgatg gccaccctcg ctctgcacga cgcacacatg
     1021 ctctcgctcc tctcgcagtc gctcacggcc atgacggtcg aagcgatggt cggccacgcc
     1081 ggctcgttcc accccttcct tcacgacgtc acgcgccctc acccgacgca gatcgaagtc
     1141 gcgggaaaca tccgcaggct cctcgaggga agccgctttg ctgtccacca tgaggaggag
     1201 gtcaaggtca aggacgacga gggcattctc cgccaggacc gctacccctt gcgcacgtct
     1261 cctcagtggc tcggcccgct cgtcagcgac ctcattcacg cccacgccgt cctcaccatc
     1321 gaggccggcc agtcgacgac cgacaaccct ctcatcgacg tcgggaacaa gacttcgcac
     1381 cacggcggca atttccaggc tgccgctgtg gccaacacca tggagaagac tcgcctcggg
     1441 ctcgcccaga tcggcaagct caacttcacg cagctcaccg agatgctcaa cgccggcatg
     1501 aaccgcggcc tcccctcctg cctcgcggcc gaagacccct cgctctccta ccactgcaag
     1561 ggcctcgaca tcgccgctgc ggcgtacacc tcggagttgg gacacctcgc caaccctgtg
     1621 acgacgcatg tccagccggc tgagatggcg aaccaggcgg tcaactcgct tgcgctcatc
     1681 tcggctcgtc gcacgaccga gtccaacgac gtcctttctc tcctcctcgc cacccacctc
     1741 tactgcgttc tccaagccat cgacttgcgc gcgatcgagt tcgagttcaa gaagcagttc
     1801 ggcccagcca tcgtctcgct catcgaccag cactttggct ccgccatgac cggctcgaac
     1861 ctgcgcgacg agctcgtcga gaaggtgaac aagacgctcg ccaagcgcct cgagcagacc
     1921 aactcgtacg acctcgtccc gcgctggcac gacgccttct ccttcgccgc cggcaccgtc
     1981 gtcgaggtcc tctcgtcgac gtcgctctcg ctcgccgccg tcaacgcctg gaaggtcgcc
     2041 gccgccgagt cggccatctc gctcacccgc caagtccgcg agaccttctg gtccgccgcg
     2101 tcgacctcgt cgcccgcgct ctcgtacctc tcgccgcgca ctcagatcct ctacgccttc
     2161 gtccgcgagg agcttggcgt caaggcccgc cgcggagacg tcttcctcgg caagcaagag
     2221 gtgacgatcg gctcgaacgt ctccaagatc tacgaggcca tcaagtcggg caggatcaac
     2281 aacgtcctcc tcaagatgct cgcttactag agccaggcat caaataaaac gaaaggctca
     2341 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2401 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//