BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_I13453 1 BBa_I13453 Pbad promoter 2005-05-24T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PBad promoter from I0500 without AraC. false false _11_ 0 253 6 In stock false true jkm BBa_K1129030 1 BBa_K1129030 Phenylalanine ammonia lyase under arabinose promoter 2013-09-15T11:00:00Z 2015-05-08T01:09:19Z Arab+rbs+PAL+term Arab+rbs+PAL+term false false _1441_ 0 9774 9 It's complicated true none false UBC iGEM 2013 component2348001 1 BBa_B0034 component2347999 1 BBa_I13453 component2348014 1 BBa_B0015 component2348007 1 BBa_K801091 annotation2348001 1 BBa_B0034 range2348001 1 139 150 annotation2347999 1 BBa_I13453 range2347999 1 1 130 annotation2348007 1 BBa_K801091 range2348007 1 157 2304 annotation2348014 1 BBa_B0015 range2348014 1 2313 2441 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K801091 1 BBa_K801091 phenylalanine ammonia lyase (PAL) coding region 2012-09-20T11:00:00Z 2015-05-08T01:13:24Z Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nuccore/AX366866 PMID: 15932991 Coding region for the enzyme phenylalanine ammonia lyase (PAL) from ''Rhodosporidium toruloides'' false false _1057_ 0 13150 9 In stock false - false Ingmar Polte annotation2202129 1 Mutation range2202129 1 1356 1356 annotation2202133 1 Deletion of Cys range2202133 1 1358 1360 annotation2202128 1 Start codon range2202128 1 1 3 annotation2202130 1 PAL range2202130 1 1 2148 annotation2202137 1 Phe instead of Ser range2202137 1 1364 1364 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I13453_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K801091_sequence 1 atggcaccctcgctcgactcgatctcgcactcgttcgcaaacggcgtcgcatccgcaaagcaggctgtcaatggcgcctcgaccaacctcgcagtcgcaggctcgcacctgcccacaacccaggtcacgcaggtcgacatcgtcgagaagatgctcgccgcgccgaccgactcgacgctcgaactcgacggctactcgctcaacctcggagacgtcgtctcggccgcgaggaagggcaggcctgtccgcgtcaaggacagcgacgagatccgctcaaagattgacaaatcggtcgagttcttgcgctcgcaactctccatgagcgtctacggcgccacgactggatttggcggatccgcagacacccgcaccgaggacgccatctcgctccagaaggctctcctcgagcaccagctctgcggtgttctcccttcgtcgttcgactcgttccgcctcggccgcggtctcgagaactcgcttcccctcgaggttgttcgcggcgccatgacaatccgcgtcaacagcttgacccgcggccactcggctgtccgcctcgtcgtcctcgaggcgctcaccaacttcctcaaccacggcatcacccccatcgtccccctccgcggcaccatctctgcgtcgggcgacctctctcctctctcctacattgcagcggccatcagcggtcacccggacagcaaggtgcacgtcgtccacgagggcaaggagaagatcctgtacgcccgcgaggcgatggcgctcttcaacctcgagcccgtcgtcctcggcccgaaggaaggtctcggtctcgtcaacggcaccgccgtctcagcatcgatggccaccctcgctctgcacgacgcacacatgctctcgctcctctcgcagtcgctcacggccatgacggtcgaagcgatggtcggccacgccggctcgttccaccccttccttcacgacgtcacgcgccctcacccgacgcagatcgaagtcgcgggaaacatccgcaggctcctcgagggaagccgctttgctgtccaccatgaggaggaggtcaaggtcaaggacgacgagggcattctccgccaggaccgctaccccttgcgcacgtctcctcagtggctcggcccgctcgtcagcgacctcattcacgcccacgccgtcctcaccatcgaggccggccagtcgacgaccgacaaccctctcatcgacgtcgggaacaagacttcgcaccacggcggcaatttccaggctgccgctgtggccaacaccatggagaagactcgcctcgggctcgcccagatcggcaagctcaacttcacgcagctcaccgagatgctcaacgccggcatgaaccgcggcctcccctcctgcctcgcggccgaagacccctcgctctcctaccactgcaagggcctcgacatcgccgctgcggcgtacacctcggagttgggacacctcgccaaccctgtgacgacgcatgtccagccggctgagatggcgaaccaggcggtcaactcgcttgcgctcatctcggctcgtcgcacgaccgagtccaacgacgtcctttctctcctcctcgccacccacctctactgcgttctccaagccatcgacttgcgcgcgatcgagttcgagttcaagaagcagttcggcccagccatcgtctcgctcatcgaccagcactttggctccgccatgaccggctcgaacctgcgcgacgagctcgtcgagaaggtgaacaagacgctcgccaagcgcctcgagcagaccaactcgtacgacctcgtcccgcgctggcacgacgccttctccttcgccgccggcaccgtcgtcgaggtcctctcgtcgacgtcgctctcgctcgccgccgtcaacgcctggaaggtcgccgccgccgagtcggccatctcgctcacccgccaagtccgcgagaccttctggtccgccgcgtcgacctcgtcgcccgcgctctcgtacctctcgccgcgcactcagatcctctacgccttcgtccgcgaggagcttggcgtcaaggcccgccgcggagacgtcttcctcggcaagcaagaggtgacgatcggctcgaacgtctccaagatctacgaggccatcaagtcgggcaggatcaacaacgtcctcctcaagatgctcgct BBa_B0034_sequence 1 aaagaggagaaa BBa_K1129030_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatggcaccctcgctcgactcgatctcgcactcgttcgcaaacggcgtcgcatccgcaaagcaggctgtcaatggcgcctcgaccaacctcgcagtcgcaggctcgcacctgcccacaacccaggtcacgcaggtcgacatcgtcgagaagatgctcgccgcgccgaccgactcgacgctcgaactcgacggctactcgctcaacctcggagacgtcgtctcggccgcgaggaagggcaggcctgtccgcgtcaaggacagcgacgagatccgctcaaagattgacaaatcggtcgagttcttgcgctcgcaactctccatgagcgtctacggcgccacgactggatttggcggatccgcagacacccgcaccgaggacgccatctcgctccagaaggctctcctcgagcaccagctctgcggtgttctcccttcgtcgttcgactcgttccgcctcggccgcggtctcgagaactcgcttcccctcgaggttgttcgcggcgccatgacaatccgcgtcaacagcttgacccgcggccactcggctgtccgcctcgtcgtcctcgaggcgctcaccaacttcctcaaccacggcatcacccccatcgtccccctccgcggcaccatctctgcgtcgggcgacctctctcctctctcctacattgcagcggccatcagcggtcacccggacagcaaggtgcacgtcgtccacgagggcaaggagaagatcctgtacgcccgcgaggcgatggcgctcttcaacctcgagcccgtcgtcctcggcccgaaggaaggtctcggtctcgtcaacggcaccgccgtctcagcatcgatggccaccctcgctctgcacgacgcacacatgctctcgctcctctcgcagtcgctcacggccatgacggtcgaagcgatggtcggccacgccggctcgttccaccccttccttcacgacgtcacgcgccctcacccgacgcagatcgaagtcgcgggaaacatccgcaggctcctcgagggaagccgctttgctgtccaccatgaggaggaggtcaaggtcaaggacgacgagggcattctccgccaggaccgctaccccttgcgcacgtctcctcagtggctcggcccgctcgtcagcgacctcattcacgcccacgccgtcctcaccatcgaggccggccagtcgacgaccgacaaccctctcatcgacgtcgggaacaagacttcgcaccacggcggcaatttccaggctgccgctgtggccaacaccatggagaagactcgcctcgggctcgcccagatcggcaagctcaacttcacgcagctcaccgagatgctcaacgccggcatgaaccgcggcctcccctcctgcctcgcggccgaagacccctcgctctcctaccactgcaagggcctcgacatcgccgctgcggcgtacacctcggagttgggacacctcgccaaccctgtgacgacgcatgtccagccggctgagatggcgaaccaggcggtcaactcgcttgcgctcatctcggctcgtcgcacgaccgagtccaacgacgtcctttctctcctcctcgccacccacctctactgcgttctccaagccatcgacttgcgcgcgatcgagttcgagttcaagaagcagttcggcccagccatcgtctcgctcatcgaccagcactttggctccgccatgaccggctcgaacctgcgcgacgagctcgtcgagaaggtgaacaagacgctcgccaagcgcctcgagcagaccaactcgtacgacctcgtcccgcgctggcacgacgccttctccttcgccgccggcaccgtcgtcgaggtcctctcgtcgacgtcgctctcgctcgccgccgtcaacgcctggaaggtcgccgccgccgagtcggccatctcgctcacccgccaagtccgcgagaccttctggtccgccgcgtcgacctcgtcgcccgcgctctcgtacctctcgccgcgcactcagatcctctacgccttcgtccgcgaggagcttggcgtcaaggcccgccgcggagacgtcttcctcggcaagcaagaggtgacgatcggctcgaacgtctccaagatctacgaggccatcaagtcgggcaggatcaacaacgtcctcctcaagatgctcgcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z