LOCUS       BBa_K1129037            1979 bp    DNA     linear       22-Dec-2024
DEFINITION  4-coumarate-CoA ligase (4CL) under arabinose promoter
ACCESSION   BBa_K1129037
VERSION     BBa_K1129037.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..159
                     /label=Start codon
     misc_feature    1840..1842
                     /label=Stop codon
     CDS             157..1839
                     /label=4CL
     CDS             157..1842
                     /label=BBa_K801093
     stem_loop       1862..1905
                     /label=stem_loop
     misc_feature    1851..1930
                     /label=BBa_B0010
     terminator      1851..1930
                     /label=BBa_B0010
     stem_loop       1946..1965
                     /label=T7 TE
     misc_feature    1972..1972
                     /label=stop
     polyA_site      1966..1979
                     /label=polya
     misc_feature    1939..1979
                     /label=BBa_B0012
     terminator      1939..1979
                     /label=BBa_B0012
     terminator      1851..1979
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg cgccacaaga acaagcagtt
      181 tctcaggtga tggagaaaca gagcaacaac aacaacagtg acgtcatttt ccgatcaaag
      241 ttaccggata tttacatccc gaaccaccta tctctccacg actacatctt ccaaaacatc
      301 tccgagttcg ccactaagcc ttgcctaatc aacggaccaa ccggccacgt gtacacttac
      361 tccgacgtcc acgtcatctc ccgccaaatc gccgccaatt ttcacaaact cggcgttaac
      421 caaaacgacg tcgtcatgct cctcctccca aactgtcccg agttcgtcct ctctttcctc
      481 gccgcctcct tccgcggcgc aaccgccacc gccgcaaacc ctttcttcac tccggcggag
      541 atagctaaac aagccaaagc ctccaacacc aaactcataa tcaccgaagc tcgttacgtc
      601 gacaaaatca aaccacttca aaacgacgac ggagtagtca tcgtctgcat cgacgacaac
      661 gaatccgtgc caatccctga aggctgcctc cgcttcaccg agttgactca gtcgacaacc
      721 gaggcatcag aagtcatcga ctcggtggag atttcaccgg acgacgtggt ggcactacct
      781 tactcctctg gcacgacggg attaccaaaa ggagtgatgc tgactcacaa gggtctagtc
      841 acgagcgttg ctcagcaagt cgacggcgag aacccgaatc tttatttcca cagcgatgac
      901 gtcatactct gtgttttgcc catgtttcat atctacgctt tgaactcgat catgttgtgt
      961 ggtcttagag ttggtgcggc gattctgata atgccgaagt ttgagatcaa tctgctattg
     1021 gagctgatcc agaggtgtaa agtgacggtg gctccgatgg ttccgccgat tgtgttggcc
     1081 attgcgaagt cttcggagac ggagaagtat gatttgagct cgataagagt ggtgaaatct
     1141 ggtgctgctc ctcttggtaa agaacttgaa gatgccgtta atgccaagtt tcctaatgcc
     1201 aaactcggtc agggatacgg aatgacggaa gcaggtccag tgctagcaat gtcgttaggt
     1261 tttgcaaagg aaccttttcc ggttaagtca ggagcttgtg gtactgttgt aagaaatgct
     1321 gagatgaaaa tagttgatcc agacaccgga gattctcttt cgaggaatca acccggtgag
     1381 atttgtattc gtggtcacca gatcatgaaa ggttacctca acaatccggc agctacagca
     1441 gagaccattg ataaagacgg ttggcttcat actggagata ttggattgat cgatgacgat
     1501 gacgagcttt tcatcgttga tcgattgaaa gaacttatca agtataaagg ttttcaggta
     1561 gctccggctg agctagaggc tttgctcatc ggtcatcctg acattactga tgttgctgtt
     1621 gtcgcaatga aagaagaagc agctggtgaa gttcctgttg catttgtggt gaaatcgaag
     1681 gattcggagt tatcagaaga tgatgtgaag caattcgtgt cgaaacaggt tgtgttttac
     1741 aagagaatca acaaagtgtt cttcactgaa tccattccta aagctccatc agggaagata
     1801 ttgaggaaag atctgagggc aaaactagca aatggattgt aatactagag ccaggcatca
     1861 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1921 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//