LOCUS       BBa_K1129042            1884 bp    DNA     linear       22-Dec-2024
DEFINITION  4-coumarate-CoA ligase (4CL) under pTET constitutive promoter
ACCESSION   BBa_K1129042
VERSION     BBa_K1129042.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..64
                     /label=Start codon
     misc_feature    1745..1747
                     /label=Stop codon
     CDS             62..1744
                     /label=4CL
     CDS             62..1747
                     /label=BBa_K801093
     stem_loop       1767..1810
                     /label=stem_loop
     misc_feature    1756..1835
                     /label=BBa_B0010
     terminator      1756..1835
                     /label=BBa_B0010
     stem_loop       1851..1870
                     /label=T7 TE
     misc_feature    1877..1877
                     /label=stop
     polyA_site      1871..1884
                     /label=polya
     misc_feature    1844..1884
                     /label=BBa_B0012
     terminator      1844..1884
                     /label=BBa_B0012
     terminator      1756..1884
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatggcgcca caagaacaag cagtttctca ggtgatggag aaacagagca acaacaacaa
      121 cagtgacgtc attttccgat caaagttacc ggatatttac atcccgaacc acctatctct
      181 ccacgactac atcttccaaa acatctccga gttcgccact aagccttgcc taatcaacgg
      241 accaaccggc cacgtgtaca cttactccga cgtccacgtc atctcccgcc aaatcgccgc
      301 caattttcac aaactcggcg ttaaccaaaa cgacgtcgtc atgctcctcc tcccaaactg
      361 tcccgagttc gtcctctctt tcctcgccgc ctccttccgc ggcgcaaccg ccaccgccgc
      421 aaaccctttc ttcactccgg cggagatagc taaacaagcc aaagcctcca acaccaaact
      481 cataatcacc gaagctcgtt acgtcgacaa aatcaaacca cttcaaaacg acgacggagt
      541 agtcatcgtc tgcatcgacg acaacgaatc cgtgccaatc cctgaaggct gcctccgctt
      601 caccgagttg actcagtcga caaccgaggc atcagaagtc atcgactcgg tggagatttc
      661 accggacgac gtggtggcac taccttactc ctctggcacg acgggattac caaaaggagt
      721 gatgctgact cacaagggtc tagtcacgag cgttgctcag caagtcgacg gcgagaaccc
      781 gaatctttat ttccacagcg atgacgtcat actctgtgtt ttgcccatgt ttcatatcta
      841 cgctttgaac tcgatcatgt tgtgtggtct tagagttggt gcggcgattc tgataatgcc
      901 gaagtttgag atcaatctgc tattggagct gatccagagg tgtaaagtga cggtggctcc
      961 gatggttccg ccgattgtgt tggccattgc gaagtcttcg gagacggaga agtatgattt
     1021 gagctcgata agagtggtga aatctggtgc tgctcctctt ggtaaagaac ttgaagatgc
     1081 cgttaatgcc aagtttccta atgccaaact cggtcaggga tacggaatga cggaagcagg
     1141 tccagtgcta gcaatgtcgt taggttttgc aaaggaacct tttccggtta agtcaggagc
     1201 ttgtggtact gttgtaagaa atgctgagat gaaaatagtt gatccagaca ccggagattc
     1261 tctttcgagg aatcaacccg gtgagatttg tattcgtggt caccagatca tgaaaggtta
     1321 cctcaacaat ccggcagcta cagcagagac cattgataaa gacggttggc ttcatactgg
     1381 agatattgga ttgatcgatg acgatgacga gcttttcatc gttgatcgat tgaaagaact
     1441 tatcaagtat aaaggttttc aggtagctcc ggctgagcta gaggctttgc tcatcggtca
     1501 tcctgacatt actgatgttg ctgttgtcgc aatgaaagaa gaagcagctg gtgaagttcc
     1561 tgttgcattt gtggtgaaat cgaaggattc ggagttatca gaagatgatg tgaagcaatt
     1621 cgtgtcgaaa caggttgtgt tttacaagag aatcaacaaa gtgttcttca ctgaatccat
     1681 tcctaaagct ccatcaggga agatattgag gaaagatctg agggcaaaac tagcaaatgg
     1741 attgtaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1801 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1861 cgggtgggcc tttctgcgtt tata
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