LOCUS       BBa_K112950             2487 bp    DNA     linear       23-Dec-2024
DEFINITION  BBb1 entry vector - specR
ACCESSION   BBa_K112950
VERSION     BBa_K112950.1  
FEATURES             Location/Qualifiers
     primer_bind     79..98
                     /label=g00101
     misc_feature    106..205
                     /label=attL2
     CDS             474..1484
                     /label=specR
     misc_feature    1498..2164
                     /label=colE1 orgin
     misc_feature    2304..2403
                     /label=attL1
     primer_bind     2412..2433
                     /label=ca998
     misc_feature    2271..2288
                     /label=M13-fwd
ORIGIN
        1 ggatcctaac tcgacgtgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
       61 ctgcggcgag cggtatcagc tcactcaaag gcggtaatca attcgaccca gctttcttgt
      121 acaaagttgg cattataaaa aataattgct catcaatttg ttgcaacgaa caggtcacta
      181 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
      241 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
      301 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
      361 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
      421 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
      481 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
      541 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
      601 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
      661 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
      721 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
      781 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
      841 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
      901 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
      961 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     1021 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     1081 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     1141 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     1201 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     1261 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     1321 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     1381 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     1441 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     1501 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     1561 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1621 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1681 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1741 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1801 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1861 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1921 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     1981 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2041 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2101 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2161 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2221 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtcctagg tgtaaaacga
     2281 cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt
     2341 cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagcag
     2401 gctccgaatt ggtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     2461 ggatgatttc tggaattcat gagatct
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