LOCUS       BBa_K112951             3210 bp    DNA     linear       22-Dec-2024
DEFINITION  BBb1 assembly vector - A/C resistance
ACCESSION   BBa_K112951
VERSION     BBa_K112951.1  
FEATURES             Location/Qualifiers
     primer_bind     79..98
                     /label=G00101
     misc_feature    106..130
                     /label=attB2
     misc_feature    180..1019
                     /label=p15A orgin
     CDS             1179..1838
                     /label=CamR
     misc_feature    1966..1999
                     /label=LoxP
     CDS             2340..2999
                     /label=AmpR
     misc_feature    3102..3126
                     /label=attB1
     primer_bind     3135..3156
                     /label=ca998
ORIGIN
        1 ggatcctaac tcgacgtgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
       61 ctgcggcgag cggtatcagc tcactcaaag gcggtaatca attcgaccca gctttcttgt
      121 acaaagtggt tgatccttac agatcccggt tatccacaga atcaggggag gcctctagaa
      181 atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc
      241 ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac
      301 caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc
      361 agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg
      421 ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg
      481 ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
      541 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga
      601 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga
      661 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
      721 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca
      781 cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca
      841 tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
      901 atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg
      961 aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag
     1021 cgctgatgtc cggcggtgcg catgccgtta agggattttg gtcatgaact agcttgatcg
     1081 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     1141 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1201 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1261 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1321 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     1381 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     1441 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     1501 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1561 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1621 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1681 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1741 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1801 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga
     1861 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     1921 ggttgccgcc gggcgttttt tattggtgag aatccaagcc tgcagataac ttcgtatagc
     1981 atacattata cgaagttatc tcgaggacgt caggtggcac ttttcgggga aatgtgcgcg
     2041 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2101 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2161 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2221 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2281 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2341 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2401 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2461 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2521 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2581 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2641 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2701 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2761 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2821 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2881 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2941 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3001 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3061 ttaaaaggat ctaggtgaag atccttgaat tgccattatc aacaagtttg tacaaaaaag
     3121 caggctccga attggtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     3181 taaggatgat ttctggaatt catgagatct
//