LOCUS       BBa_K112954             3344 bp    DNA     linear       25-Nov-2024
DEFINITION  BBb1 assembly vector - K/A
ACCESSION   BBa_K112954
VERSION     BBa_K112954.1  
FEATURES             Location/Qualifiers
     primer_bind     79..98
                     /label=g00101
     misc_feature    106..130
                     /label=attB2
     misc_feature    208..1019
                     /label=p15A origin
     CDS             1161..1820
                     /label=AmpR
     misc_feature    2163..2196
                     /label=LoxP
     CDS             2351..3166
                     /label=KanR
     misc_feature    3236..3260
                     /label=attB1
     primer_bind     3269..3290
                     /label=ca998
ORIGIN
        1 ggatcctaac tcgacgtgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
       61 ctgcggcgag cggtatcagc tcactcaaag gcggtaatca attcgaccca gctttcttgt
      121 acaaagtggt tgatccttac agatcccggt tatccacaga atcaggggag gcctctagaa
      181 atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc
      241 ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac
      301 caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc
      361 agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg
      421 ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg
      481 ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
      541 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga
      601 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga
      661 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
      721 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca
      781 cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca
      841 tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
      901 atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg
      961 aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag
     1021 cgctgatgtc cggcggtgcg catgccgtta agggattttg gtcatgaact agcaaggatc
     1081 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1141 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1201 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1261 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1321 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1381 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1441 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1501 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1561 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1621 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1681 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1741 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1801 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1861 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1921 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1981 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2041 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2101 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcctctgc
     2161 agataacttc gtatagcata cattatacga agttatctcg agctgatcct tcaactcagc
     2221 aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc
     2281 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     2341 aaggggtgtt atgagccata ttcaacggga aacgtcttgc tccaggccgc gattaaattc
     2401 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2461 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2521 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2581 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2641 caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2701 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2761 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2821 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2881 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg
     2941 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3001 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3061 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3121 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3181 ttggttgtaa cactggcaga gcattacgct gacttgacgg gaattgccat tatcaacaag
     3241 tttgtacaaa aaagcaggct ccgaattggt atcacgaggc agaatttcag ataaaaaaaa
     3301 tccttagctt tcgctaagga tgatttctgg aattcatgag atct
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