LOCUS       BBa_K112955             3149 bp    DNA     linear       22-Nov-2024
DEFINITION  BBb1 assembly vector - C/K
ACCESSION   BBa_K112955
VERSION     BBa_K112955.1  
FEATURES             Location/Qualifiers
     primer_bind     79..98
                     /label=g00101
     misc_feature    106..130
                     /label=attB2
     misc_feature    812..1019
                     /label=p15A origin
     CDS             1130..1945
                     /label=KanR
     misc_feature    2100..2133
                     /label=LoxP
     misc_feature    2261..2920
                     /label=CamR
     misc_feature    3041..3065
                     /label=attB1
     primer_bind     3074..3095
                     /label=ca998
ORIGIN
        1 ggatcctaac tcgacgtgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
       61 ctgcggcgag cggtatcagc tcactcaaag gcggtaatca attcgaccca gctttcttgt
      121 acaaagtggt tgatccttac agatcccggt tatccacaga atcaggggag gcctctagaa
      181 atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc
      241 ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac
      301 caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc
      361 agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg
      421 ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg
      481 ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
      541 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga
      601 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga
      661 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
      721 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca
      781 cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca
      841 tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
      901 atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg
      961 aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag
     1021 cgctgatgtc cggcggtgcg catgccgtta agggattttg gtcatgaact agctcccgtc
     1081 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact
     1141 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     1201 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     1261 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     1321 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     1381 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     1441 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     1501 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     1561 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     1621 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     1681 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     1741 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     1801 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     1861 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag
     1921 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1981 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     2041 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagctgcaga
     2101 taacttcgta tagcatacat tatacgaagt tatctcgagg cttggattct caccaataaa
     2161 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     2221 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     2281 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     2341 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     2401 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     2461 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     2521 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     2581 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     2641 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     2701 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     2761 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     2821 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2881 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2941 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     3001 tggaacctct tacgtgcccg atcaagaatt gccattatca acaagtttgt acaaaaaagc
     3061 aggctccgaa ttggtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     3121 aaggatgatt tctggaattc atgagatct
//