LOCUS       BBa_K112956             3147 bp    DNA     linear       22-Dec-2024
DEFINITION  BBb1 assembly vector - K/C
ACCESSION   BBa_K112956
VERSION     BBa_K112956.1  
FEATURES             Location/Qualifiers
     primer_bind     79..98
                     /label=g00101
     misc_feature    106..130
                     /label=attB2
     misc_feature    208..1019
                     /label=p15A origin
     CDS             1179..1838
                     /label=CamR
     misc_feature    1966..1999
                     /label=LoxP
     CDS             2154..2969
                     /label=KanR
     misc_feature    3039..3063
                     /label=attB1
     primer_bind     3072..3093
                     /label=ca998
ORIGIN
        1 ggatcctaac tcgacgtgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
       61 ctgcggcgag cggtatcagc tcactcaaag gcggtaatca attcgaccca gctttcttgt
      121 acaaagtggt tgatccttac agatcccggt tatccacaga atcaggggag gcctctagaa
      181 atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc
      241 ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac
      301 caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc
      361 agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg
      421 ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg
      481 ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
      541 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga
      601 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga
      661 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
      721 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca
      781 cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca
      841 tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
      901 atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg
      961 aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag
     1021 cgctgatgtc cggcggtgcg catgccgtta agggattttg gtcatgaact agcttgatcg
     1081 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     1141 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1201 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1261 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1321 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     1381 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     1441 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     1501 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1561 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1621 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1681 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1741 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1801 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga
     1861 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     1921 ggttgccgcc gggcgttttt tattggtgag aatccaagcc tgcagataac ttcgtatagc
     1981 atacattata cgaagttatc tcgagctgat ccttcaactc agcaaaagtt cgatttattc
     2041 aacaaagcca cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2101 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     2161 atattcaacg ggaaacgtct tgctccaggc cgcgattaaa ttccaacatg gatgctgatt
     2221 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat
     2281 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     2341 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     2401 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     2461 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     2521 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     2581 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     2641 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     2701 ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     2761 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     2821 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     2881 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     2941 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     3001 agagcattac gctgacttga cgggaattgc cattatcaac aagtttgtac aaaaaagcag
     3061 gctccgaatt ggtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     3121 ggatgatttc tggaattcat gagatct
//