LOCUS       BBa_K113002             1817 bp    DNA     linear       26-Dec-2024
DEFINITION  pLac->cI434->pT7->cI434 operator ->GFP
ACCESSION   BBa_K113002
VERSION     BBa_K113002.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    712..717
                     /label=2
     CDS             82..717
                     /label=cI 434
     CDS             82..717
                     /label=BBa_C0056
     stem_loop       737..780
                     /label=stem_loop
     misc_feature    726..805
                     /label=BBa_B0010
     terminator      726..805
                     /label=BBa_B0010
     stem_loop       821..840
                     /label=T7 TE
     misc_feature    847..847
                     /label=stop
     polyA_site      841..854
                     /label=polya
     misc_feature    814..854
                     /label=BBa_B0012
     terminator      814..854
                     /label=BBa_B0012
     misc_feature    947..950
                     /label=conserved
     RBS             943..954
                     /label=BBa_B0034
     stem_loop       1700..1743
                     /label=stem_loop
     misc_feature    1689..1768
                     /label=BBa_B0010
     terminator      1689..1768
                     /label=BBa_B0010
     stem_loop       1784..1803
                     /label=T7 TE
     misc_feature    1810..1810
                     /label=stop
     polyA_site      1804..1817
                     /label=polya
     misc_feature    1777..1817
                     /label=BBa_B0012
     terminator      1777..1817
                     /label=BBa_B0012
     promoter        863..885
                     /label=T7 promoter
     misc_feature    889..934
                     /label=BBa_R0052
     misc_feature    943..954
                     /label=BBa_B0034
     misc_feature    961..1680
                     /label=BBa_E0040
     misc_feature    1689..1768
                     /label=BBa_B0010
     misc_feature    1777..1817
                     /label=BBa_B0012
     misc_feature    863..1817
                     /label=BBa_K113001
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagtatt tcttccaggg taaaaagcaa aagaatccag
      121 cttggactta accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag
      181 cagctcgaaa acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt
      241 ggcgtaagtg ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg
      301 cacgttgagc ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt
      361 gaagcttgtg aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac
      421 ttattaggca atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt
      481 caaagcatcc ctgaaggtca tatggtgtta gtagatactg gacgggagcc agtgaatgga
      541 agccttgttg tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata
      601 gatggcggtc agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac
      661 ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt ataataatac
      721 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      781 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      841 tttctgcgtt tatatactag agtaatacga ctcactatag ggagaccctt gacaaacaag
      901 atacattgta tgaaaataca agaaagtttg ttgatactag agaaagagga gaaatactag
      961 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     1021 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     1081 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     1141 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
     1201 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     1261 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     1321 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     1381 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     1441 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     1501 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1561 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1621 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
     1681 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1741 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1801 gcctttctgc gtttata
//