BBa_K113004
1
BBa_K113004
pT7->tetR operator->GFP
2008-10-25T11:00:00Z
2015-05-08T01:09:20Z
T7 promoter is from BBa_I719015.
tetR operator is the same as BBa_R0040.
RBS + GFP + T7 terminator is from BBa_I719015.
tetR operator is inserted between T7 promoter and RBS in BBa_I719015, which is a T7 promoter-regulated GFP sequence. Thus tetR can repress the expression of GFP once the concentration of tetR reaches certain level.
false
false
_254_
0
2711
9
It's complicated
false
For repressor test (right side).----HKUSTers/2008
false
WU Jingjing, Wang Jinyu
annotation1988818
1
BBa_E0040
range1988818
1
100
819
annotation1988820
1
BBa_B0010
range1988820
1
828
907
annotation1988817
1
BBa_B0034
range1988817
1
82
93
annotation1988819
1
GFP protein
range1988819
1
100
819
annotation1988821
1
BBa_B0012
range1988821
1
916
956
annotation1988815
1
BBa_I719005
range1988815
1
1
23
annotation1988816
1
tetR operator
range1988816
1
27
73
BBa_K113004_sequence
1
taatacgactcactatagggagaccctccctatcagtgatagagattgacatccctatcagtgatagagatactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z