LOCUS       BBa_K113005             1867 bp    DNA     linear       27-Dec-2024
DEFINITION  pLac->tetR->pT7->tetR operator ->GFP
ACCESSION   BBa_K113005
VERSION     BBa_K113005.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..701
                     /label=tetR
     misc_feature    702..735
                     /label=SsrA
     misc_feature    742..766
                     /label=Help:Barcodes
     CDS             82..766
                     /label=BBa_C0040
     misc_feature    1..766
                     /label=BBa_I730002
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    775..854
                     /label=BBa_B0010
     terminator      775..854
                     /label=BBa_B0010
     stem_loop       870..889
                     /label=T7 TE
     misc_feature    896..896
                     /label=stop
     polyA_site      890..903
                     /label=polya
     misc_feature    863..903
                     /label=BBa_B0012
     terminator      863..903
                     /label=BBa_B0012
     terminator      775..903
                     /label=BBa_B0015
     misc_feature    1..903
                     /label=BBa_K113003
     misc_feature    997..1000
                     /label=conserved
     RBS             993..1004
                     /label=BBa_B0034
     stem_loop       1750..1793
                     /label=stem_loop
     misc_feature    1739..1818
                     /label=BBa_B0010
     terminator      1739..1818
                     /label=BBa_B0010
     stem_loop       1834..1853
                     /label=T7 TE
     misc_feature    1860..1860
                     /label=stop
     polyA_site      1854..1867
                     /label=polya
     misc_feature    1827..1867
                     /label=BBa_B0012
     terminator      1827..1867
                     /label=BBa_B0012
     misc_feature    912..934
                     /label=BBa_I719005
     misc_feature    938..984
                     /label=tetR operator
     misc_feature    993..1004
                     /label=BBa_B0034
     misc_feature    1011..1730
                     /label=BBa_E0040
     CDS             1011..1730
                     /label=GFP protein
     misc_feature    1739..1818
                     /label=BBa_B0010
     misc_feature    1827..1867
                     /label=BBa_B0012
     misc_feature    912..1867
                     /label=BBa_K113004
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
      121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
      181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
      241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
      301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
      361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
      421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
      481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
      541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
      601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
      661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
      721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc
      781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      901 atatactaga gtaatacgac tcactatagg gagaccctcc ctatcagtga tagagattga
      961 catccctatc agtgatagag atactactag agaaagagga gaaatactag atgcgtaaag
     1021 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     1081 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     1141 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     1201 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
     1261 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     1321 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     1381 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     1441 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
     1501 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     1561 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     1621 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     1681 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
     1741 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1801 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1861 gtttata
//