BBa_K113012
1
BBa_K113012
weaken overlapping T7 promoter
2008-10-25T11:00:00Z
2015-05-08T01:09:20Z
The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
Two T7 promoters are reversely overlapping in two base pairs while a point mutation occurs at the 12th base pair, from A-T to T-A.
false
false
_254_
0
2711
9
It's complicated
false
It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
false
Wu Jingjing, Wang Jinyu
annotation1989402
1
T7 promoter
range1989402
1
20
37
annotation1989400
1
T7 promoter
range1989400
1
4
21
BBa_K113012_sequence
1
cccctatagagagtcgtattaatacgactctctatagggg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z