LOCUS       BBa_K113018             1792 bp    DNA     linear       22-Nov-2024
DEFINITION  weaken overlapping T7 promoter->GFP/RFP
ACCESSION   BBa_K113018
VERSION     BBa_K113018.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     CDS             136..855
                     /label=GFP protein
     CDS             136..855
                     /label=BBa_E0040
     misc_feature    868..871
                     /label=conserved
     RBS             864..875
                     /label=BBa_B0034
     promoter        887..904
                     /label=T7 promoter 
     promoter        903..920
                     /label=T7 promoter
     promoter        884..923
                     /label=BBa_K113012
     misc_feature    936..939
                     /label=conserved
     RBS             932..943
                     /label=BBa_B0034
     CDS             950..1624
                     /label=mrfp1
     misc_feature    1631..1655
                     /label=Help:Barcodes
     CDS             950..1655
                     /label=BBa_E1010
     stem_loop       1675..1718
                     /label=stem_loop
     misc_feature    1664..1743
                     /label=BBa_B0010
     terminator      1664..1743
                     /label=BBa_B0010
     stem_loop       1759..1778
                     /label=T7 TE
     misc_feature    1785..1785
                     /label=stop
     polyA_site      1779..1792
                     /label=polya
     misc_feature    1752..1792
                     /label=BBa_B0012
     terminator      1752..1792
                     /label=BBa_B0012
     terminator      1664..1792
                     /label=BBa_B0015
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      181 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      241 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      301 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      361 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      421 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      481 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      541 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      601 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      661 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      721 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      781 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      841 ctatacaaat aataatacta gagaaagagg agaaatacta gagcccctat agagagtcgt
      901 attaatacga ctctctatag gggtactaga gaaagaggag aaatactaga tggcttcctc
      961 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
     1021 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
     1081 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
     1141 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
     1201 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
     1261 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
     1321 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
     1381 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
     1441 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
     1501 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
     1561 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
     1621 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
     1681 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1741 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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