LOCUS BBa_K113019 4779 bp DNA linear 22-Nov-2024 DEFINITION pBad/araC->T7 polymerase->GFP ACCESSION BBa_K113019 VERSION BBa_K113019.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1219..3896 /label=BBa_I712069 misc_feature 3909..3912 /label=conserved RBS 3905..3916 /label=BBa_B0034 CDS 3923..4642 /label=GFP protein CDS 3923..4642 /label=BBa_E0040 stem_loop 4662..4705 /label=stem_loop misc_feature 4651..4730 /label=BBa_B0010 terminator 4651..4730 /label=BBa_B0010 stem_loop 4746..4765 /label=T7 TE misc_feature 4772..4772 /label=stop polyA_site 4766..4779 /label=polya misc_feature 4739..4779 /label=BBa_B0012 terminator 4739..4779 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagcc accatgaaca cgattaacat cgctaagaac gacttctctg 1261 acatcgaact ggctgctatc ccgttcaaca ctctggctga ccattacggt gagcgtttag 1321 ctcgcgaaca gttggccctt gagcatgagt cttacgagat gggtgaagca cgcttccgca 1381 agatgtttga gcgtcaactt aaagctggtg aggttgcgga taacgctgcc gccaagcctc 1441 tcatcactac cctactccct aagatgattg cacgcatcaa cgactggttt gaggaagtga 1501 aagctaagcg cggcaagcgc ccgacagcct tccagttcct gcaagaaatc aagccggaag 1561 ccgtagcgta catcaccatt aagaccactc tggcttgcct aaccagtgct gacaatacaa 1621 ccgttcaggc tgtagcaagc gcaatcggtc gggccattga ggacgaggct cgcttcggtc 1681 gtatccgtga ccttgaagct aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc 1741 gcgtagggca cgtctacaag aaagcattta tgcaagttgt cgaggctgac atgctctcta 1801 agggtctact cggtggcgag gcgtggtctt cgtggcataa ggaagactct attcatgtag 1861 gagtacgctg catcgagatg ctcattgagt caaccggaat ggttagctta caccgccaaa 1921 atgctggcgt agtaggtcaa gactctgaga ctatcgaact cgcacctgaa tacgctgagg 1981 ctatcgcaac ccgtgcaggt gcgctggctg gcatctctcc gatgttccaa ccttgcgtag 2041 ttcctcctaa gccgtggact ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc 2101 ctctggcgct ggtgcgtact cacagtaaga aagcactgat gcgctacgaa gacgtttaca 2161 tgcctgaggt gtacaaagcg attaacattg cgcaaaacac cgcatggaaa atcaacaaga 2221 aagtcctagc ggtcgccaac gtaatcacca agtggaagca ttgtccggtc gaggacatcc 2281 ctgcgattga gcgtgaagaa ctcccgatga aaccggaaga catcgacatg aatcctgagg 2341 ctctcaccgc gtggaaacgt gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt 2401 ctcgccgtat cagccttgag ttcatgcttg agcaagccaa taagtttgct aaccataagg 2461 ccatctggtt cccttacaac atggactggc gcggtcgtgt ttacgctgtg tcaatgttca 2521 acccgcaagg taacgatatg accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg 2581 gtaaggaagg ttactactgg ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg 2641 ttccgttccc tgagcgcatc aagttcattg aggaaaacca cgagaacatc atggcttgcg 2701 ctaagtctcc actggagaac acttggtggg ctgagcaaga ttctccgttc tgcttccttg 2761 cgttctgctt tgagtacgct ggggtacagc accacggcct gagctataac tgctcccttc 2821 cgctggcgtt tgacgggtct tgctctggca tccagcactt ctccgcgatg ctccgagatg 2881 aggtaggtgg tcgcgcggtt aacttgcttc ctagtgaaac cgttcaggac atctacggga 2941 ttgttgctaa gaaagtcaac gagattctac aagcagacgc aatcaatggg accgataacg 3001 aagtagttac cgtgaccgat gagaacactg gtgaaatctc tgagaaagtc aagctgggca 3061 ctaaggcact ggctggtcaa tggctggctt acggtgttac tcgcagtgtg actaagcgtt 3121 cagtcatgac gctggcttac gggtccaaag agttcggctt ccgtcaacaa gtgctggaag 3181 ataccattca gccagctatt gattccggca agggtctgat gttcactcag ccgaatcagg 3241 ctgctggata catggctaag ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg 3301 ttgaagcaat gaactggctt aagtctgctg ctaagctgct ggctgctgag gtcaaagata 3361 agaagactgg agagattctt cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt 3421 tccctgtgtg gcaggaatac aagaagccta ttcagacgcg cttgaacctg atgttcctcg 3481 gtcagttccg cttacagcct accattaaca ccaacaaaga tagcgagatt gatgcacaca 3541 aacaggagtc tggtatcgct cctaactttg tacacagcca agacggtagc caccttcgta 3601 agactgtagt gtgggcacac gagaagtacg gaatcgaatc ttttgcactg attcacgact 3661 ccttcggtac cattccggct gacgctgcga acctgttcaa agcagtgcgc gaaactatgg 3721 ttgacacata tgagtcttgt gatgtactgg ctgatttcta cgaccagttc gctgaccagt 3781 tgcacgagtc tcaattggac aaaatgccag cacttccggc taaaggtaac ttgaacctcc 3841 gtgacatctt agagtcggac ttcgcgttcg cggatccaaa aaagaagaga aaggtatact 3901 agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc 3961 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg 4021 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact 4081 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag 4141 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt 4201 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa 4261 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga 4321 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat 4381 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga 4441 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt 4501 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga 4561 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat 4621 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt 4681 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 4741 acactggctc accttcgggt gggcctttct gcgtttata //