##gff-version 3
##sequence-region BBa_K113021 1 7322
BBa_K113021	.	ribosome_entry_site	5616	5627	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K113021	.	conserved_region	5620	5623	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K113021	.	terminator	4042	4170	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K113021	.	terminator	4042	4121	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K113021	.	stem_loop	4053	4096	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K113021	.	engineered_region	4042	4121	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K113021	.	terminator	4130	4170	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K113021	.	engineered_region	4130	4170	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K113021	.	stop_codon	4163	4163	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K113021	.	stem_loop	4137	4156	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K113021	.	polyA_site	4157	4170	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K113021	.	terminator	7194	7322	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K113021	.	terminator	7194	7273	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K113021	.	stem_loop	7205	7248	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K113021	.	engineered_region	7194	7273	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K113021	.	terminator	7282	7322	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K113021	.	engineered_region	7282	7322	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K113021	.	stop_codon	7315	7315	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K113021	.	stem_loop	7289	7308	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K113021	.	polyA_site	7309	7322	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K113021	.	promoter	5636	5681	.	+	0	ID=BBa_R0052;Name=BBa_R0052
BBa_K113021	.	promoter	5637	5642	.	+	0	ID=annotation2031;Name=-35;Parent=BBa_R0052
BBa_K113021	.	non_covalent_binding_site	5643	5656	.	+	0	ID=annotation2027;Name=OR2;Parent=BBa_R0052
BBa_K113021	.	promoter	5659	5664	.	+	0	ID=annotation2030;Name=-10;Parent=BBa_R0052
BBa_K113021	.	non_covalent_binding_site	5636	5678	.	+	0	ID=annotation2026;Name=OR2;Parent=BBa_R0052
BBa_K113021	.	engineered_region	5636	5681	.	+	0	ID=annotation7068;Name=BBa_R0052;Parent=BBa_R0052
BBa_K113021	.	promoter	5636	5641	.	+	0	ID=annotation2029;Name=-35;Parent=BBa_R0052
BBa_K113021	.	start_codon	5670	5672	.	+	0	ID=annotation2032;Name=start;Parent=BBa_R0052
BBa_K113021	.	non_covalent_binding_site	5665	5678	.	+	0	ID=annotation2028;Name=OR1;Parent=BBa_R0052
BBa_K113021	.	CDS	4177	4896	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K113021	.	CDS	4177	4896	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K113021	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K113021	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K113021	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K113021	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K113021	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K113021	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K113021	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K113021	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K113021	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K113021	.	ribosome_entry_site	4905	4916	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K113021	.	conserved_region	4909	4912	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K113021	.	CDS	4923	5607	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K113021	.	engineered_tag	5583	5607	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K113021	.	tag	5543	5576	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K113021	.	CDS	4926	5542	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K113021	.	CDS	5818	6453	.	+	0	ID=BBa_C0056;Name=BBa_C0056
BBa_K113021	.	CDS	5818	6453	.	+	0	ID=annotation1063931;Name=cI 434;Parent=BBa_C0056
BBa_K113021	.	stop_codon	6448	6453	.	+	0	ID=annotation1063930;Name=2;Parent=BBa_C0056
BBa_K113021	.	engineered_region	138	2815	.	+	0	ID=BBa_I712069;Name=BBa_I712069
BBa_K113021	.	promoter	2824	4033	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_K113021	.	promoter	3866	3937	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_K113021	.	promoter	3903	4033	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_K113021	.	sequence_feature	2824	3902	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_K113021	.	ribosome_entry_site	6462	6473	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K113021	.	conserved_region	6466	6469	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K113021	.	ribosome_entry_site	5800	5811	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K113021	.	conserved_region	5804	5807	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K113021	.	CDS	6480	7185	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K113021	.	CDS	6480	7154	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K113021	.	engineered_tag	7161	7185	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K113021	.	promoter	5738	5791	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K113021	.	promoter	5757	5762	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K113021	.	non_covalent_binding_site	5763	5781	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K113021	.	engineered_region	5738	5791	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K113021	.	non_covalent_binding_site	5738	5756	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K113021	.	promoter	5780	5785	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K113021	.	promoter	5690	5729	.	+	0	ID=BBa_K113010;Name=BBa_K113010
BBa_K113021	.	promoter	5709	5726	.	+	0	ID=annotation1989307;Name=T7 promoter;Parent=BBa_K113010
BBa_K113021	.	promoter	5693	5710	.	+	0	ID=annotation1989277;Name=T7 promoter;Parent=BBa_K113010
>BBa_K113021
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaccatgaacacgattaaca
tcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgttt
agctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaa
cttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgca
tcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagcc
ggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgta
gcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaaga
aaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacat
gctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgc
atcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgaga
ctatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttcca
accttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcg
ctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaaca
ttgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtcc
ggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctc
accgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttca
tgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgttta
cgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggt
aaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatca
agttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaaga
ttctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctccctt
ccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcgg
ttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcaga
cgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctg
ggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctgg
cttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaaggg
tctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggta
gctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggag
agattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctat
tcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagatt
gatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtag
tgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgc
gaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccag
ttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgaca
tcttagagtcggacttcgcgttcgcggatccaaaaaagaagagaaaggtatactagagttatgacaacttgacggctac
atcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaa
tagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcct
ggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacgg
cgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcc
tcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagca
gatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatg
cggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtag
gcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctg
gcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggt
gagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgtt
aaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatac
tcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctc
gctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaac
aaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttt
tatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggc
tagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagatgcgtaaagg
agaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagt
ggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccat
ggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactt
tttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgt
gctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaaca
ttcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaa
agttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaatt
ggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaaga
gagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataata
ctagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatg
aggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgt
aaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaa
ggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaa
aagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaagg
tttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagag
catcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattat
ttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatg
tgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta
ctagagaaagaggagaaatactagagttgacaaacaagatacattgtatgaaaatacaagaaagtttgttgatactaga
gcccctatagtgagtcgtattaatacgactcactataggggtactagagtccctatcagtgatagagattgacatccct
atcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaa
aagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaa
aacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatg
gcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagc
tggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttatta
ggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatgg
tgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacatt
caagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacgga
aactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataatactagagaaagaggagaaatacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata