BBa_K1131000
1
BBa_K1131000
Flavin-containing monooxygenase (FMO); M. aminisulfidivorans
2013-08-18T11:00:00Z
2015-05-08T01:09:20Z
This part comes from the M. aminisulfidivorans genome. We obtained it however from Han et. al 2011 - (Bio-indigo production in two different fermentation systems using recombinant Escherichia coli cells harboring a flavin-containing monooxygenase gene (fmo)).
This Flavin-containing monooxygenase (FMO) from M. aminisulfidivorans can be expressed in many strains of E. Coli to produce indigo dye. In the presence of indole and oxygen, FMO can catalyze the addition of a hydroxyl group to indole generating the intermediate indoxyl. Indoxyl then naturally oxidizes to generate indigo which, due to its hydrophobicity, crashes out of solution. The part submitted is the ORF of FMO only.
false
false
_1443_
0
16618
9
It's complicated
false
-
false
Ramya Prathuri
BBa_K1131000_sequence
1
atggccactaggattgctatcttaggtgctggtccttctggaatggctcaattgagagcatttcagtctgcccaggagaagggtgccgaaattcctgagttagtctgcttcgaaaagcaggccgattggggtggtcaatggaattacacatggaggaccggattggacgaaaatggtgaacctgtacattcatctatgtatagatatttgtggtctaacggacctaaagagtgcttggagtttgccgattatacctttgatgaacattttggaaaaccaatcgcatcttacccaccaagagaagtattatgggactacattaagggtagagttgagaaagctggtgttaggaaatatatcagatttaataccgccgttagacatgtcgaatttaacgaggactcacaaaccttcaccgttactgtccaggaccataccactgacactatatattcagaagagtttgattacgttgtttgttgcactggtcatttctctaccccatacgttccagaatttgagggatttgagaagttcggtggaagaatattgcatgcccacgattttagagatgccttagagtttaaagacaagaccgttttattagtaggatcttcatactcagctgaagacatcggttctcaatgttacaaatacggtgccaagaagttaatttcttgttatagaacagcaccaatgggatataaatggcctgagaactgggatgaaagaccaaatttggttagggtcgacacagaaaatgcctactttgctgatggttcttctgagaaagtagatgctattatattatgtacaggttacattcatcacttcccattcttaaatgatgacttgaggttagttactaacaacaggttatggccattaaatttatataagggagtagtatgggaggataaccctaagttcttctacattggaatgcaggatcagtggtattcattcaatatgtttgacgcacaggcttggtatgctagggatgtaatcatgggtagattacctttgccatctaaggaggaaatgaaagctgattcaatggcttggagagaaaaggagttaacattggttaccgctgaggagatgtatacataccagggtgactatattcagaatttgatcgacatgactgattatccatctttcgatatccctgccaccaataaaacctttcttgagtggaaacatcataagaaagaaaacattatgacattcagggaccattcatataggtcattaatgacaggtactatggcacctaaacaccatactccatggattgacgccttggatgattcattagaagcttacttatctgacaagtcagaaatccctgttgccaaggaggctggatcctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z