BBa_K1131002 1 BBa_K1131002 B-Glucosidase; B. Circulans 2013-09-10T11:00:00Z 2015-05-08T01:09:20Z edit The beta-Glucosidase from B. Circulans cleaves the glycosidic linkage of our substrate of interest, indican, via hydrolysis. This enzyme is expressed well in E. Coli and shows activity with a variety of substrates including indican and X-Gal, offering a potential alternative in the latter case to the lacZ beta-galactosidase in a blue-white screen. This sequence corresponds to the ORF of the enzyme only. false false _1443_ 0 16618 9 It's complicated false -- false Ramya Prathuri BBa_K1131002_sequence 1 atgtctatccatatgtttccatctgattttaagtggggtgttgctacagcagcttatcaaatcgagggtgcttacaacgaagacggaaggggtatgtcaatttgggacacttttgctcacacaccaggtaaagtcaagaatggtgacaacggtaacgttgcatgcgattcttatcatagagttgaagaagatgttcaattattaaaagacttaggtgttaaagtttacagattttctatttcttggccaagagtcttgcctcagggaaccggtgaggttaatagagcaggtttggactactatcacagattagtcgatgaattgttagccaacggaattgagccattctgcacattgtatcattgggatttgccacaagcattgcaagaccaaggtggttggggttctaggataacaatagacgcctttgctgaatatgctgaattaatgtttaaggagttaggtggtaagattaaacagtggatcacttttaatgagccatggtgtatggcattcttgtctaactatttgggtgttcacgcacctggtaacaaggatttacaattagccattgatgtttcacatcatttattagtagcccacggaagggcagtcacattattcagggaattgggaatatctggagaaatcggtatcgcccctaacacatcttgggcagtcccttacagaagaacaaaagaagacatggaggcctgtttgagagtcaacggatggtctggtgactggtatttagaccctatctacttcggtgagtacccaaagttcatgttggattggtacgaaaatttgggttataagccacctattgttgatggtgatatggaattaattcatcagcctatagactttattggtattaactattatacttcatcaatgaatagatacaatcctggtgaagcaggtggtatgttgtcttctgaagcaatatcaatgggagcccctaaaacagatataggttgggaaatatatgctgaaggtttatatgatttgttgagatacacagctgataaatacggaaatccaaccttatacataacagaaaacggagcatgttataacgacggattatcattagatggaagaatacatgatcaaagaagaatcgattatttagcaatgcatttaatccaagcctcaagggcaatcgaagatggaattaatttgaagggatacatggaatggtcattaatggacaattttgaatgggctgaaggatatggtatgagatttggtttggttcatgttgattatgatacattagttagaactcctaaggattcattttactggtataaaggtgttatttcaagaggatggttggatttataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z