BBa_K1131005
1
BBa_K1131005
mutant oleD 'ASP' glucosyltransferase; S. antibiotics
2013-09-12T11:00:00Z
2015-05-08T01:09:20Z
The source of this part was a plasmid donated from the Anderson lab and subsequently modified. The 'ASP' mutation comes from Thorston et. al. 2010
This is the mutant version of oleD glucosyltransferase part BBa_K1131004. Mutations identified by Thorston et. al. 2010 at P67T, S132F, and A242V were introduced to oleD generating the 'ASP' version of the protein. OleD 'ASP' is reported to have a much larger set of substrates it can glucosylate compared to the non mutated oleD.
false
false
_1443_
0
16618
9
It's complicated
false
The part has an additional 15 amino acids at the C-terminus and has the same sequence as uniprot Q53685, except with the 3 mutations described above.
false
Ramya Prathuri
BBa_K1131005_sequence
1
atggcggccgcttctaggctaccacccagaccactcccgcccacatcgccatgttctccatcgccgcccacggccatgtgaaccccagcctggaggtgatccgtgaactcgtcgcccgcggccaccgggtcacgtacgccattccgcccgtcttcgccgacaaggtggccgccaccggcccccggcccgtcctctaccactccaccctgcccggcaccgacgccgacccggaggcatggggaagcaccctgctggacaaccgtcgcaccttcctgaacgacgcgatccaggcgctcccgcagctcgccgatgcctacgccgacgacatccccgatctcgtcctgcacgacatcacctcctacccggcccgcgtcctggcccgccgctggggcgtcccggcggtctccctcttcccgaacctcgtcgcctggaagggttacgaggaggaggtcgccgagccgatgtggcgcgaaccccggcagaccgagcgcggacgggcctactacgcccggttcgaggcatggctgaaggagaacgggatcaccgagcacccggacacgttcgccagtcatccgccgcgctccctggtgctcatcccgaaggcgctccagccgcacgccgaccgggtggacgaagacgtgtacaccttcgtcggcgcctgccagggagaccgcgccgaggaaggcggctggcagcggcccgccggcgcggagaaggtcgtcctggtgtcgctcggctcggtgttcaccaagcagcccgccttctaccgggagtgcgtgcgcgccttcgggaacctgcccggctggcacctcgtcctccagatcggccggaaggtgacccccgccgaactgggggagctgccggacaacgtggaggtgcacgactgggtgccgcagctcgcgatcctgcgccaggccgatctgttcgtcacccacgcgggcgccggcggcagccaggaggggctggccaccgcgacgcccatgatcgccgtaccgcaggccgtcgaccagttcggcaacgccgacatgctccaagggctcggcgtcgcccggaagctggcgaccgaggaggccaccgccgacctgctccgcgagaccgccctcgctctggtggacgacccggaggtcgcgcgccggctccggcgaatccaggcggagatggcccaggagggcggcacccggcgggcggccgacctcatcgaggccgaactgcccgcgcgccacgagcggcaggagccggtgggcgaccgacccaacgtgggcgatcgtccggcgggcgtgcgttccgatcgtcagcgttccgcgctgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z