##gff-version 3
##sequence-region BBa_K1132012 1 2403
BBa_K1132012	.	engineered_region	1564	2403	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K1132012	.	terminator	2275	2354	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1132012	.	stem_loop	2286	2329	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1132012	.	engineered_region	2275	2354	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1132012	.	ribosome_entry_site	1564	1575	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1132012	.	conserved_region	1568	1571	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1132012	.	terminator	2363	2403	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1132012	.	engineered_region	2363	2403	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1132012	.	stop_codon	2396	2396	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1132012	.	polyA_site	2390	2403	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1132012	.	stem_loop	2370	2389	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1132012	.	CDS	1582	2241	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1132012	.	tag	2202	2235	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1132012	.	engineered_tag	2242	2266	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1132012	.	CDS	1585	2201	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1132012	.	CDS	1	1555	.	+	0	ID=BBa_K1132011;Name=BBa_K1132011
BBa_K1132012	.	engineered_region	760	1555	.	+	0	ID=BBa_K081017;Name=BBa_K081017;Parent=BBa_K1132011
BBa_K1132012	.	CDS	781	1555	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_K081017
BBa_K1132012	.	CDS	781	1530	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_K1132012	.	engineered_tag	1531	1555	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_K1132012	.	ribosome_entry_site	760	774	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081017
BBa_K1132012	.	engineered_region	760	774	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1132012	.	ribosome_entry_site	760	774	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1132012	.	ribosome_entry_site	767	771	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1132012	.	CDS	1	751	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_K1132011
BBa_K1132012	.	engineered_tag	727	751	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
>BBa_K1132012
atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtca
aatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccat
ggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagc
ctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatg
tggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatct
ttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaattt
gccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccg
ccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgat
caaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacc
tccgaaggctaataacgctgatagtgctagtgtagatcgctactagagattaaagaggagaaatactagatggccgtca
ctgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgc
tagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaa
aagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaag
ggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccgg
ccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaa
tcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatatt
gtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattg
tcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaa
caacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagcc
aagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagaaagaggagaaatacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata