##gff-version 3
##sequence-region BBa_K1132013 1 2465
BBa_K1132013	.	engineered_region	1	56	.	+	0	ID=BBa_K608003;Name=BBa_K608003
BBa_K1132013	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K608003
BBa_K1132013	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1132013	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1132013	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1132013	.	promoter	1	35	.	+	0	ID=BBa_J23104;Name=BBa_J23104;Parent=BBa_K608003
BBa_K1132013	.	CDS	63	2465	.	+	0	ID=BBa_K1132012;Name=BBa_K1132012
BBa_K1132013	.	engineered_region	1626	2465	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K1132012
BBa_K1132013	.	terminator	2337	2416	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1132013	.	stem_loop	2348	2391	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1132013	.	engineered_region	2337	2416	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1132013	.	ribosome_entry_site	1626	1637	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1132013	.	conserved_region	1630	1633	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1132013	.	CDS	1644	2303	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1132013	.	tag	2264	2297	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1132013	.	engineered_tag	2304	2328	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1132013	.	CDS	1647	2263	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1132013	.	terminator	2425	2465	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1132013	.	stem_loop	2432	2451	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1132013	.	stop_codon	2458	2458	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1132013	.	polyA_site	2452	2465	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1132013	.	engineered_region	2425	2465	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1132013	.	CDS	63	1617	.	+	0	ID=BBa_K1132011;Name=BBa_K1132011;Parent=BBa_K1132012
BBa_K1132013	.	engineered_region	822	1617	.	+	0	ID=BBa_K081017;Name=BBa_K081017;Parent=BBa_K1132011
BBa_K1132013	.	CDS	843	1617	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_K081017
BBa_K1132013	.	CDS	843	1592	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_K1132013	.	engineered_tag	1593	1617	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_K1132013	.	ribosome_entry_site	822	836	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081017
BBa_K1132013	.	ribosome_entry_site	829	833	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1132013	.	engineered_region	822	836	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1132013	.	ribosome_entry_site	822	836	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1132013	.	CDS	63	813	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_K1132011
BBa_K1132013	.	engineered_tag	789	813	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
>BBa_K1132013
ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaagtactagatgagtgtcaacttagc
ttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggc
gttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaa
aatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgca
attctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaa
gtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattc
tcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacga
aaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggat
gaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcatta
tggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacg
ctgatagtgctagtgtagatcgctactagagattaaagaggagaaatactagatggccgtcactgatttaagtttgacc
aattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttac
ccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaa
tcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccac
tgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtg
cggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctagg
ccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtccc
agcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttg
ccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatga
taaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgtt
atccaataataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgtccagattagata
aaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgccca
gaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgag
atgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaa
gttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatga
aactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtg
gggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactg
atagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcct
tgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgcttta
gtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata