##gff-version 3
##sequence-region BBa_K1132016 1 3216
BBa_K1132016	.	engineered_region	117	890	.	+	0	ID=BBa_K081014;Name=BBa_K081014
BBa_K1132016	.	CDS	138	843	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K1132016	.	CDS	138	812	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1132016	.	engineered_tag	819	843	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1132016	.	terminator	852	890	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K1132016	.	polyA_site	883	890	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1132016	.	stem_loop	861	882	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1132016	.	polyA_site	852	860	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1132016	.	engineered_region	852	890	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1132016	.	ribosome_entry_site	117	131	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K1132016	.	engineered_region	117	131	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1132016	.	ribosome_entry_site	124	128	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1132016	.	ribosome_entry_site	117	131	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1132016	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K1132016	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K1132016	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K1132016	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K1132016	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K1132016	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K1132016	.	engineered_region	961	3216	.	+	0	ID=BBa_K592018;Name=BBa_K592018
BBa_K1132016	.	CDS	979	3216	.	+	0	ID=BBa_K592000;Name=BBa_K592000;Parent=BBa_K592018
BBa_K1132016	.	CDS	979	3216	.	+	0	ID=annotation2132074;Name=cph8;Parent=BBa_K592000
BBa_K1132016	.	ribosome_entry_site	961	972	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592018
BBa_K1132016	.	conserved_region	965	968	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1132016	.	promoter	899	952	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1132016	.	engineered_region	899	952	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1132016	.	non_covalent_binding_site	899	917	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1132016	.	promoter	918	923	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1132016	.	non_covalent_binding_site	924	942	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1132016	.	promoter	941	946	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_K1132016
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagattaaagaggagaaatactagatggcttcctccgaagacgtt
atcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtg
aaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacat
cctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcc
ttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccc
tgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaa
aaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctg
aaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtg
cttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctga
aggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgcccc
tgacagggcggggtttttttttactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactga
gcactactagagaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaa
ccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcag
ccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgttt
gatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttggg
cgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaact
cgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgc
caacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgc
tataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcct
gcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtt
tatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgc
gcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaagga
cggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaa
ttttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcgg
agcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcct
gttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgag
aaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaa
tttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgct
ctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagac
ggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaat
cggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatat
ggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctg
acgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaag
agtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaa
tgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcatt
gaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggct
gggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccgga
acaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggca
attgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcg
cctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataa