LOCUS BBa_K1132017 5689 bp DNA linear 26-Dec-2024 DEFINITION For the characterisation of the red light responsive system, under tetR regulation ACCESSION BBa_K1132017 VERSION BBa_K1132017.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23104 RBS 44..56 /label=RBS-3\Weak RBS 50..53 /label=RBS misc_feature 44..56 /label=BBa_B0032 RBS 44..56 /label=BBa_B0032 misc_feature 1..56 /label=BBa_K608003 misc_feature 789..813 /label=Help:Barcodes CDS 63..813 /label=BBa_I15008 RBS 822..836 /label=RBS-1\Strong RBS 829..833 /label=RBS misc_feature 822..836 /label=BBa_B0030 RBS 822..836 /label=BBa_B0030 CDS 843..1592 /label=PcyA misc_feature 1593..1617 /label=Help:Barcodes CDS 843..1617 /label=BBa_I15009 misc_feature 822..1617 /label=BBa_K081017 CDS 63..1617 /label=BBa_K1132011 misc_feature 1630..1633 /label=conserved RBS 1626..1637 /label=BBa_B0034 CDS 1647..2263 /label=tetR misc_feature 2264..2297 /label=SsrA misc_feature 2304..2328 /label=Help:Barcodes CDS 1644..2303 /label=BBa_C0040 stem_loop 2348..2391 /label=stem_loop misc_feature 2337..2416 /label=BBa_B0010 terminator 2337..2416 /label=BBa_B0010 stem_loop 2432..2451 /label=T7 TE misc_feature 2458..2458 /label=stop polyA_site 2452..2465 /label=polya misc_feature 2425..2465 /label=BBa_B0012 terminator 2425..2465 /label=BBa_B0012 misc_feature 1626..2465 /label=BBa_P0440 CDS 63..2465 /label=BBa_K1132012 CDS 1..2465 /label=BBa_K1132013 misc_feature 2486..2503 /label=C1 OmpR misc_feature 2507..2524 /label=C2 OmpR misc_feature 2527..2544 /label=C3 OmpR promoter 2548..2553 /label=-35 promoter 2571..2576 /label=-10 promoter 2474..2581 /label=BBa_R0082 RBS 2590..2604 /label=RBS-1\Strong RBS 2597..2601 /label=RBS misc_feature 2590..2604 /label=BBa_B0030 RBS 2590..2604 /label=BBa_B0030 CDS 2611..3285 /label=mrfp1 misc_feature 3292..3316 /label=Help:Barcodes CDS 2611..3316 /label=BBa_E1010 misc_feature 3325..3363 /label=B1006 stem_loop 3334..3355 /label=modified thr terminator polyA_site 3356..3363 /label=PolyA polyA_site 3325..3333 /label=PolyA terminator 3325..3363 /label=BBa_B1006 misc_feature 2590..3363 /label=BBa_K081014 misc_feature 3372..3390 /label=TetR 1 misc_feature 3372..3425 /label=BBa_R0040 promoter 3391..3396 /label=-35 misc_feature 3397..3415 /label=TetR 2 promoter 3414..3419 /label=-10 promoter 3372..3425 /label=BBa_R0040 misc_feature 3438..3441 /label=conserved RBS 3434..3445 /label=BBa_B0034 CDS 3452..5689 /label=cph8 CDS 3452..5689 /label=BBa_K592000 misc_feature 3434..5689 /label=BBa_K592018 CDS 2474..5689 /label=BBa_K1132016 ORIGIN 1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagtcacaca ggaaagtact 61 agatgagtgt caacttagct tcccagttgc gggaagggac gaaaaaatcc cactccatgg 121 cggagaacgt cggctttgtc aaatgcttcc tcaagggcgt tgtcgagaaa aattcctacc 181 gtaagctggt tggcaatctc tactttgtct acagtgccat ggaagaggaa atggcaaaat 241 ttaaggacca tcccatcctc agccacattt acttccccga actcaaccgc aaacaaagcc 301 tagagcaaga cctgcaattc tattacggct ccaactggcg gcaagaagtg aaaatttctg 361 ccgctggcca agcctatgtg gaccgagtcc ggcaagtggc cgctacggcc cctgaattgt 421 tggtggccca ttcctacacc cgttacctgg gggatctttc cggcggtcaa attctcaaga 481 aaattgccca aaatgccatg aatctccacg atggtggcac agctttctat gaatttgccg 541 acattgatga cgaaaaggct tttaaaaata cctaccgtca agctatgaat gatctgccca 601 ttgaccaagc caccgccgaa cggattgtgg atgaagccaa tgacgccttt gccatgaaca 661 tgaaaatgtt caacgaactt gaaggcaacc tgatcaaggc gatcggcatt atggtgttca 721 acagcctcac ccgtcgccgc agtcaaggca gcaccgaagt tggcctcgcc acctccgaag 781 gctaataacg ctgatagtgc tagtgtagat cgctactaga gattaaagag gagaaatact 841 agatggccgt cactgattta agtttgacca attcttccct gatgcctacg ttgaacccga 901 tgattcaaca gttggccctg gcgatcgccg ctagttggca aagtttaccc ctcaagccct 961 atcaattgcc ggaggatttg ggctacgtag aaggccgcct ggaaggggaa aagttagtga 1021 ttgaaaatcg gtgctaccaa acgccccagt ttcgcaaaat gcatttggag ttggccaagg 1081 tgggcaaagg gttggatatt ctccactgtg taatgtttcc tgagccttta tacggtctac 1141 ctttgtttgg ctgtgacatt gtggccggcc ccggtggagt aagtgcggct attgcggatc 1201 tatcccccac ccaaagcgat cgccaattgc ccgcagcgta ccaaaaatca ttggcagagc 1261 taggccagcc agaatttgag caacaacggg aattgccccc ctggggagaa atattttctg 1321 aatattgttt attcatccgt cccagcaatg tcactgaaga agaaagattt gtacaaaggg 1381 tagtggactt tttgcaaatt cattgtcacc aatccatcgt tgccgaaccc ttgtctgaag 1441 ctcaaacttt ggagcaccgt caggggcaaa ttcattactg ccaacaacaa cagaaaaatg 1501 ataaaacccg tcgggtactg gaaaaagctt ttggggaagc ttgggcggaa cggtatatga 1561 gccaagtctt atttgatgtt atccaataat aactctgata gtgctagtgt agatctctac 1621 tagagaaaga ggagaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg 1681 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga 1741 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg 1801 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg 1861 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc 1921 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc 1981 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg 2041 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag 2101 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag 2161 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga 2221 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa 2281 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag 2341 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2401 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2461 ttatatacta gagtcccttg catttacatt ttgaaacatc tatagcgata aatgaaacat 2521 cttaaaagtt ttagtatcat attcgtgttg gattattctg catttttggg gagaatggac 2581 ttactagaga ttaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag 2641 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa 2701 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa 2761 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa 2821 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt 2881 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac 2941 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg 3001 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg 3061 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt 3121 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg 3181 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc 3241 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag 3301 tgctagtgta gatcgctact agagaaaaaa aaaccccgcc cctgacaggg cggggttttt 3361 ttttactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 3421 agcactacta gagaaagagg agaaatacta gatggccacc accgtacaac tcagcgacca 3481 atccctccgt cagctagaaa ccctcgccat ccacaccgcc cacctgattc agccccacgg 3541 tttagtggtg gtcctgcaag aaccagacct caccatcagc caaattagcg ccaactgcac 3601 cggcatttta gggcgatcgc cagaggattt gttgggcaga accctagggg aagtgtttga 3661 tagctttcag attgatccca tccagagtcg cctaacggcc ggacaaatca gcagcctcaa 3721 ccccagtaaa ctttgggcgc gggtcatggg ggacgacttt gtcatttttg acggggtttt 3781 tcatcgcaac agtgacggtt tattggtatg tgaactcgag ccagcctaca cttccgataa 3841 tctgcccttc ctcggttttt atcacatggc caacgctgcc ctgaatcggt tgcgccaaca 3901 agctaatcta cgggatttct acgatgttat tgtcgaagaa gtccgccgta tgactggctt 3961 tgaccgggtg atgctatacc gctttgatga aaataaccac ggtgatgtca ttgccgaaga 4021 taaacgggat gatatggaac cctatttggg cctgcactat cccgaatcgg atattcccca 4081 acccgcccgt cggctattta tccacaaccc cattcgagta attcccgatg tttatggtgt 4141 ggcggtgccc ctgaccccag cggttaaccc cagcaccaac cgagcggtgg atttaacaga 4201 atccattctg cgcagtgcgt accattgcca cttgacctat ctgaaaaata tgggggtagg 4261 agcgtcttta accatttccc taattaagga cggccatctc tgggggctca ttgcctgcca 4321 ccatcaaacc cccaaagtaa ttccctttga actgcgtaaa gcctgcgaat tttttggtcg 4381 ggtggtgttt agcaacattt ccgcccagga agatacggaa accttcgatt accgggtgca 4441 gctggcggag catgaagcgg ttttattgga caaaatgacc acggcggcgg attttgtcga 4501 aggattaact aatcatcccg atcgcctgtt gggattaacg ggctcccagg gggcggccat 4561 ttgctttggg gaaaaattga ttttagtagg ggaaaccccg gacgagaaag cagtgcaata 4621 tttactgcaa tggttggaga atcgggaagt gcaagacgtt ttcttcacct cttccctctc 4681 acaaatttat cctgatgcag tgaattttaa atccgtggcc agtggcttat tggccattcc 4741 cattgcccgt cacaactttt tgctctggtt tcgccctgaa gtgttgcaaa cggttaattg 4801 gggcggtgac ccaaatcatg cttacgaagc tacccaggaa gacggtaaaa tcgagctcca 4861 tccccgccaa tcctttgacc tctggaaaga aattgtccga ctccaatctt tgccctggca 4921 atcggtggaa atccaaagtg ccctggccct gaaaaaggcg atcgtcaacc tcattttgcg 4981 ccaggcagaa gaattgcata tggcggctgg tgttaagcaa ctggcggatg accgcacgct 5041 gctgatggcg ggggtaagtc acgacttgcg cacgccgctg acgcgtattc gcctggcgac 5101 tgagatgatg agcgagcagg atggctatct ggcagaatcg atcaataaag atatcgaaga 5161 gtgcaacgcc atcattgagc agtttatcga ctacctgcgc accgggcagg agatgccgat 5221 ggaaatggcg gatcttaatg cagtactcgg tgaggtgatt gctgccgaaa gtggctatga 5281 gcgggaaatt gaaaccgcgc tttaccccgg cagcattgaa gtgaaaatgc acccgctgtc 5341 gatcaaacgc gcggtggcga atatggtggt caacgccgcc cgttatggca atggctgggt 5401 caaagtcagc agcggaacgg agccgaatcg cgcctggttc caggtggaag atgacggtcc 5461 gggaattgcg ccggaacaac gtaagcacct gttccagccg tttgtccgcg gcgacagtgc 5521 gcgcaccatt agcggcacgg gattagggct ggcaattgtg cagcgtatcg tggataacca 5581 taacgggatg ctggagcttg gcaccagcga gcggggcggg ctttccattc gcgcctggct 5641 gccagtgccg gtaacgcggg cgcagggcat gacaaaagaa gggtaataa //