LOCUS       BBa_K1132018            1032 bp    DNA     linear       22-Nov-2024
DEFINITION  FixK2 promoter-rbs-mRFP-term
ACCESSION   BBa_K1132018
VERSION     BBa_K1132018.1  
FEATURES             Location/Qualifiers
     promoter        1..250
                     /label=FixK2 promoter
     promoter        1..250
                     /label=BBa_K592006
     RBS             259..273
                     /label=RBS-1\Strong
     RBS             266..270
                     /label=RBS
     misc_feature    259..273
                     /label=BBa_B0030
     RBS             259..273
                     /label=BBa_B0030
     CDS             280..954
                     /label=mrfp1
     misc_feature    961..985
                     /label=Help:Barcodes
     CDS             280..985
                     /label=BBa_E1010
     misc_feature    994..1032
                     /label=B1006
     stem_loop       1003..1024
                     /label=modified thr terminator
     polyA_site      1025..1032
                     /label=PolyA
     polyA_site      994..1002
                     /label=PolyA
     terminator      994..1032
                     /label=BBa_B1006
     misc_feature    259..1032
                     /label=BBa_K081014
ORIGIN
        1 gccggagccg attatccgca cccgtccttg gtcttggaca cactcgctcg cgatccgaag
       61 ccgcctttca aagatactcc gcagtgaaac gcatctttta agcgcgactt gtcgccttcg
      121 cgcagcagaa cactcgtatg gcactcaatc catgatcgct tcgttcgctc cgacgcgcgt
      181 tgcggggccc ccctcgaccg gatgacaaca cattgcgtca cctcaactgc tgcgcgcgca
      241 ctgcgtcaca tactagagat taaagaggag aaatactaga tggcttcctc cgaagacgtt
      301 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
      361 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
      421 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
      481 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
      541 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
      601 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
      661 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
      721 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
      781 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
      841 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
      901 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
      961 cgctgatagt gctagtgtag atcgctacta gagaaaaaaa aaccccgccc ctgacagggc
     1021 ggggtttttt tt
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