##gff-version 3
##sequence-region BBa_K1132018 1 1032
BBa_K1132018	.	promoter	1	250	.	+	0	ID=BBa_K592006;Name=BBa_K592006
BBa_K1132018	.	promoter	1	250	.	+	0	ID=annotation2130179;Name=FixK2 promoter;Parent=BBa_K592006
BBa_K1132018	.	engineered_region	259	1032	.	+	0	ID=BBa_K081014;Name=BBa_K081014
BBa_K1132018	.	terminator	994	1032	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K1132018	.	polyA_site	994	1002	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1132018	.	engineered_region	994	1032	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1132018	.	polyA_site	1025	1032	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1132018	.	stem_loop	1003	1024	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1132018	.	ribosome_entry_site	259	273	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K1132018	.	ribosome_entry_site	259	273	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1132018	.	ribosome_entry_site	266	270	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1132018	.	engineered_region	259	273	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1132018	.	CDS	280	985	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K1132018	.	engineered_tag	961	985	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1132018	.	CDS	280	954	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_K1132018
gccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactc
cgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcg
cttcgttcgctccgacgcgcgttgcggggcccccctcgaccggatgacaacacattgcgtcacctcaactgctgcgcgc
gcactgcgtcacatactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgc
gtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacga
aggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttc
cagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttca
aatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagtt
catctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaa
gcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtg
gtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacat
caaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccacc
ggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgcccctgacagggcggggttt
ttttt